In vitro neurogenesis of adult neural stem cells from bone marrow and brain
LicenseStandard (Fassung vom 03.05.2003)
This document contains a dissertation written to obtain the degree "Dr. rer. med." of the Medical Faculty of the University of Ulm. The scope of my thesis has been to identify possible alternative cell sources for neural stem cells which fulfill important requirements for the use of these cells in regenerative treatment strategies: (i) Generation of high yields of cells out of a small starting population without loosing their differentiation potential, (ii) on-demand availability of cells without major logistical and ethical problems and (iii) the possibility to standardize the cell source in a future clinical setting. Isolation and characterization of human NPCs from adult human hippocampus derived from epileptic surgery procedures (selective hippocampectomy or medio-temporal lobectomy) served as positive control. A second approach has been the identification and in vitro characterization of neural stem cells in the adult midbrain as a cell source for possible endogenous regeneration in the brain region where the neurodegenerative process in Parkinson’s disease is located. Since the reported data on in vivo dopaminergic neurogenesis in the adult brain are conflicting, the aim of the second part of this study was to investigate dopaminergic neurogenesis from adult neural stem cells in vitro to provide data on adult dopaminergic differentiation of neural stem cells as one major prerequisite for endogenous regenerative approaches in Parkinson’s disease by recruiting endogenous stem cells in the adult brain.
Subject HeadingsNeurogenese [GND]
Neural stem cells [MeSH]
Parkinson disease [MeSH]