Pathophysiologische Bedeutung von Interleukin-1ß und Interleukin-18 bei akuter Pankreatitis: eine tierexperimentelle Studie am Modell der Caerulein-induzierten Pankreatitis
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Caspase-1 activation has been shown to be of central importance in the pathogenesis of severe acute pancreatitis. IL-1ß and IL-18 are two major substrates cleaved and activated by caspase-1. IL-1ß is well known to mediate a detrimental effect in AP but little is known about the role of IL-18 in this context. Materials and methods: cerulein induced pancreatitis in male C57BL6 mice. Mice were treated with saline, irrelevant IgG, neutralising anti IL-1ß and anti IL-18 antibodies. We assessed intrapancreatic extent of necrosis, leukocyte infiltration, quantitative IL-1ß, IL18 and caspase-1 mRNA expression as well as systemic LDH, lipase, amylase IL-1ß and IL-18 release. Results: treatment with anti IL-1ß and IL-18 antibody significantly reduced the intrapancreatic extent of necrosis and leukocyte infiltration compared with saline and IgG treated mice. In both anti IL-1ß and anti IL-18 treated groups only intrapancreatic IL-18 mRNA upregulation was significantly attenuated. In terms of systemic parameters IL-18 concentrations were significantly decreased in the anti IL-18 group only.
Subject HeadingsBauchspeicheldrüsenentzündung [GND]
Interleukin 18 [GND]
Interleukin 1-beta [GND]