Nachweis und Charakterisierung somatischer Hypermutationen des Immunglobulin-Schwerkettengens primärer mediastinaler B-Zell-Lymphome
LicenseStandard (Fassung vom 03.05.2003)
Primary mediastinal B-cell lymphoma (PMBL) is a subentity of diffuse large B-cell lymphomas (DLBCL) originating from the human thymus. The investigation of rearranged immunoglobulin heavy-chain genes gives information about the differentiation stage of the lymphomagenic B-cell. 13 cases of PMBL were analyzed by polymerase chain reaction (PCR) to detect monoclonality and subsequently were cloned and analyzed by DNA sequencing to detect somatic hypermutations and possible intraclonal diversity (ongoing mutations). All cases showed a high rate of somatic hypermutation (average 13,0%). In 12 cases the rearrangements were potentially functional. No ongoing mutations were detected in all cases except for the formerly generated B-cell line MedB-1. These findings support the separation of PMBL from other DLBCL as a distinct subentity.
Subject HeadingsB-Zell-Lymphom [GND]
Lymphoma, B-cell [MeSH]