• English
    • Deutsch
View Item 
  •   OPARU Home
  • Medizinische Fakultät
  • Publikationen
  • View Item
  •   OPARU Home
  • Medizinische Fakultät
  • Publikationen
  • View Item
  • English 
    • English
    • Deutsch
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Characterization of the Leukemia Initiating Cell in Human Acute Myeloid Leukemia

Thumbnail
Download
vts_9461_14273.pdf (2.597Mb)
Veröffentlichung
2016-07-01
DOI
10.18725/OPARU-4241
Dissertation


Authors
Ihme, Erika Ruth Susann
Faculties
Medizinische Fakultät
License
Standard
https://oparu.uni-ulm.de/xmlui/license_v3
Abstract
The aim of this thesis was to establish a miRNA profile of functionally validated LICs from patients suffering from AML. To achieve this, multicolor staining protocols were established to highly purify sorted LIC and non-LIC populations from patients with AML as well for sorting their normal counterparts from bone marrow of healthy donors. In a second step, functional validation of the LIC properties was achieved by performing successfully transplantations into the immunocompromised xenograft NSG mouse model. In this study 22 de novo AML samples were included and sorted for their leukemic stem- and progenitor subpopulations as well as the more differentiated leukemic CD34- fraction. In total 11 patient samples were able to form a human leukemia graft in NSG mice with a tendency of higher engraftment in FLT3 mutated and aberrant karyotype patients. By performing next generation sequencing analysis, a miRNA signature of these validated LICs was established performing comparative analysis between the LIC and the leukemic non-LIC fraction or the normal human bone marrow counterparts. Therefore, this study is the first one that has established a miRNA expression pattern in in vivo validated AML LICs.
Date created
2014
Subject Headings
Acute myeloid leukemia [MeSH]
Leukemia [MeSH]
Keywords
fluorescence activated cell sorter (FACS); granulocyte-macrophage progenitor (GMP); hematopoietic stem cell; leukemia initiating cell (LIC); lymphoid-primed multipotent progenitor (LMPP); miRNA; stem cell; xenotransplantation
Dewey Decimal Group
DDC 610 / Medicine & health

Metadata
Show full item record

Citation example

Ihme, Erika Ruth Susann (2016): Characterization of the Leukemia Initiating Cell in Human Acute Myeloid Leukemia. Open Access Repositorium der Universität Ulm. Dissertation. http://dx.doi.org/10.18725/OPARU-4241

Other citation formats



About OPARU | Contact Us
Impressum | Privacy statement
 

 

Advanced Search

Browse

All of OPARUCommunities & CollectionsFacultiesInstitutionsPersonsResource typesUlm SerialsDewey Decimal ClassesFundingThis CollectionFacultiesInstitutionsPersonsResource typesUlm SerialsDewey Decimal ClassesFunding

My Account

LoginRegister

Statistics

View Usage Statistics

About OPARU | Contact Us
Impressum | Privacy statement