Etablierung und Charakterisierung neuer Chordomzelllinien
Auch gedruckt in der BibliothekW: W-H 14.503
Goerttler, Lukas Tsepo
Ressourcen- / MedientypDissertation, Text
Datum der Freischaltung2015-12-14
Aims: Chordoma is a tumour derived from chorda remnants which expresses early embryonal stem cell factors such as members of the sonic hedgehog pathway. We aimed at establishing new chordoma cell lines to define characteristic molecular patterns by gene expression profiling, thereby focusing on the sonic hedgehog pathway. Methods: Gene expression profiling was done by Agilent Human GE 4x44K Microarray. Selected targets were evaluated by PCR, Western blotting, immunocytology, and histology. Results: We established three new chordoma cell-lines (U-CH3, U-CH6, U-CH7, U-CH10) in addition to the published chordoma cell-lines U-CH1, U-CH2, and MUG-Chor1. The cell lines highly expressed brachyury, EMA, S100, and vimentin at protein and RNA levels. The seven cell lines clustered together when compared to 60 NCI cell lines (derived from carcinomas of breast, ovary, lung, kidney, prostate, and colon; melanoma, central nervous tumors, leukaemias). In all chordoma cell lines, sonic hedgehog (SHH) and smoothened (SMO) were highly expressed on RNA and protein levels. In addition, 30 chordomas showed a consistent expression of SHH and SMO in situ. Conclusion: The new chordoma cell lines serve as models for further analysis of this rare tumour entity and should foster research for successful treatment strategies. The sonic hedgehog reveals himself to be deficient.
SHH protein, human
Microarray gene expression profiling