Die Auswirkung von inhalativ verabreichtem H2S auf die Entzündungsantwort nach einem stumpfen Thoraxtrauma
The treatment of acute lung injury and septic complications after blunt chest trauma remains a challenge. Inhaled hydrogen sulfide (H2S) may cause a hibernation-like metabolic state, which refers to an attenuated systemic inflammatory response. Therefore, we tested the hypothesis that inhaled H2S-induced suspended animation may attenuate the inflammation after pulmonary contusion. Male Sprague Dawley rats were subjected to blunt chest trauma (blast wave) or sham procedure and subsequently exposed to a continuous flow of H2S (100 ppm) or control gas for 6 hours. Body temperature and activity were measured by an implanted transmitter. At 6, 24 or 48 hours after trauma animals were sacrificed and the cellular contents of bronchoalveolar lavage (BAL) as well as cytokine concentrations in BAL, plasma and culture supernatants of blood mononuclear cells, Kupffer cells and splenic macrophages were determined. H2S inhalation caused a significant reduction in body temperature and activity. The trauma-induced increase in alveolar macrophage counts was abrogated 48 h after trauma when animals received H2S, whereas the trauma-induced increase in neutrophil counts was unaltered. Furthermore, H2S inhalation partially attenuated the mediator release in BAL and culture supernatants of Kupffer cells as well as splenic macrophages, it altered plasma cytokine concentrations but did not affect the trauma-induced changes in mononuclear cell culture supernatants. These findings indicate that inhaled H2S induced a reduced metabolic expenditure and partially attenuated inflammation after trauma. Nevertheless, in contrast to hypoxic- or pathogen-induced lung injury, H2S treatment appears to have no protective effect after blunt chest trauma.
Erstellung / Fertigstellung
Normierte SchlagwörterBrustkorbverletzung [GND]
Hydrogen sulfide [MeSH]
Thoracic injuries [MeSH]