Vergleichende histologische und immunhistologische Untersuchungen der Gewebsreaktionen auf polymere Implantate im Tiermodell
Auch gedruckt in der BibliothekW: W-H 12.843
Ressourcen- / MedientypDissertation, Text
Datum der Freischaltung2012-02-27
A novel biodegradable polymeric implant material (Polyepsiloncaprolacton-Butylacrylat, PCL-BA) was used for the reconstruction of a complete defect of the gastric wall in Sprague-Dawley-Rats (n = 42). ). In the control group the defect of the gastric wall was closed by primary wound closure (n = 21). In the baseline group (n = 21) the animals were kept under same conditions without any surgical procedure. This group was used for reference values. In another implantation group the gastric wall defect was closed by using Poly-L-Lactid (PLL). These experiments had to interrupt after 15 animals (n = 15) because of serious complications like perforation of the gastric wall, fistula and peritonitis. The implantation time was 7 days, 4 weeks and 6 months in all animals. In the PCL-BA and in the control group as well as in the baseline group no complications were detected. After explantation different parameters of the immune respone (C5b, C3b, CD2, CD14, CD68 and IgG) have been detected by immunhistological staining with following computer-based picture analysis. In the PCL-BA group a histological verified complete regeneration of the gastric wall was found. A regeneration by scar formation was detected in the control group. The complete regeneration of the gastric wall in the PCL-BA group was correlated with high inflammation detected by high level of C5b, C3b, CD2, CD14 and CD68 after 1 week of implantation time and following clear decrease of the inflammation after 4 weeks and 6 months. In the PLL-group a delayed increase of the inflammation was detected after 4 weeks with continued high inflammation parameters after 6 months. After showing the chemical, bacteriological and enzymatical stability of PCL-BA under the conditions of the stomach the implantat material seems to be adequate for the reconstruction of the upper aerodigestive tract. In a next step the use of PCL-BA for the reconstruction of the pharynx in a large animal experiment is planned.