Heparansulfatproteoglykane bei mesenchymalen Stammzellen und reifen differenzierten Zellen
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Heparan sulfate proteoglycans are important extracellular matrix components. Their binding to a variety of other matrix components and growth factors is believed to be important in tissue development by participating in processes such as growth and differentiation. However, information on the expression of heparan sulfate proteoglycans by mesenchymal stem cells, which have the ability to develop into multiple mesenchymal tissue types, and adult cell types, is still very limited. In order to understand the role of heparan sulfate proteoglycans in differentiation processes, it is important to know their expression in mesenchymal stem cells and in different adult cell types. Therefore the expression of some heparan sulfate proteoglycan core proteins in human mesenchymal stem cells, osteoblasts, chondrocytes and adipocytes was examined. In addition to determine changes in heparan sulfate proteoglycan expression while osteogenic differentiation, the expression in mesenchymal stem cells and osteogenic differentiated cells (derived from mesenchymal stem cells by treating with osteogenic differentiation medium over 28 days) was compared. It could be shown that many heparan sulfate proteoglycans are expressed in mesenchymal stem cells and adult cells with in some cases only minor variations in quantity. But there are also differences. Glypican-3, for example, showed a strong expression in adipocytes, whereas expression in mesenchymal stem cells and osteoblasts was almost undetectable. The comparison of mesenchymal stem cells and osteogenic differentiated cells was interesting too. Whereas expression of some of the heparan sulfate proteoglycan core proteins remained essentially constant during osteogenic differentiation, others exhibited remarkable changes, e.g. betaglycan showed a strong up-regulation. These results suggest a participation of heparan sulfate proteoglycans in differentiation processes.
Subject HeadingsMesenchymzelle [GND]
Heparan sulfate proteoglycans [MeSH]
Mesenchymal stem cells [MeSH]