Untersuchung forensisch relevanter SNPs mittels Minisequencing und Kapillarelektrophorese
Schell, David Christian
LizenzStandard (Fassung vom 01.10.2008)
Many different methods have been established for SNP detection and especially minisequencing has often been used. For our study we have selected 5 Y-chromosomal SNPs (M9, M17, M45, M170, M173) based on the degree of polymorphism. PCR primers were designed with the aim to get amplicon lengths of < 200 bp. The Y-SNPs were optimized in singleplex reactions and combined to multiplex approaches by sequential optimization. Finally, we were able to analyse these 5 Y-chromosomal SNPs in one PCR/SNaPshot reaction. To validate this method for forensic stain analysis we investigated bones, bloodstains, cigarette butts and epithelial cells and mixed stain containing male-female DNA. Additionally, we typed 40 unrelated Caucasian individuals to obtain our regional haplotype frequencies.
Erstellung / Fertigstellung
Normierte SchlagwörterDegradation [GND]
Gerichtliche Wissenschaften [GND]
Forensic pathology [MeSH]