Bedeutung des Autocrine Motility Factor Receptor für den transformierten Phänotyp von humanen Papillenkarzinomzellen (AVC1)
LicenseStandard (Fassung vom 01.10.2008)
In this dissertation it could be shown that to the Autocrine Motility Factor Receptor (AMFR), before described for other tumors, comes an important role with processes of the tumor progression in the carcinoma of the ampulla vateri. The identification of the gene took place via cDNA array technology by means of comparative expression profile analyses of numerous tissue samples from pancreas (healthy and inflammatory pancreas as well as pancreatic carcinoma) and carcinoma of the ampulla vateri. Here the AMFR showed up as a gene highly expressed in carcinoma of the ampulla vateri, in addition the AMFR took a key role with the differentiation between inflammatory and malignant processes in the peripancreatic area. Subsequently, this expression profile could be proved by qRT PCR analyses and Northern Blot analyses. The expression profile of the ligand AMF correlated only in parts with the profile of its receptor. To characterize the influence of the AMFR on processes of the tumor progression and metastasis a human cell line of carcinoma of the ampulla vateri "AVC1", established by Sorio et al., has been consulted. This cell line showed a strong primary expression of the receptor in the Northern Blot. The repression of the AMFR expression by the use of siRNA permitted a direct correlation of potential changes by comparing the strong expression with the repressed expression. The accomplished in vitro experiments to proliferation and substrate-independent growth showed a statistically significant decline of these qualities after repression of the AMFR. The final characterization of these processes requires further analysis. In particular by establishing an in vivo xenograft mouse model with the available AVC1 cell line more detailed findings are expected, concerning connections between AMFR and the ligand AMF, as well as associated processes between tumor cells and its stroma.
Subject HeadingsPapillom [GND]
Autocrine motility factor [MeSH]
Receptors, cytokine [MeSH]