Analyse des genomischen Status der Proto-Onkogene c-Rel und BCL11A sowie deren RNA- und Proteinexpression in Marginalzonen-B-Zell-Lymphomen und diffus großzelligen B-Zell-Lymphomen des Gastrointestinaltraktes
Dissertation
Faculties
Medizinische FakultätAbstract
Amplifications of the c-Rel locus on chromosome 2p13-p16 have been described in distinct lymphoma subtypes, most notably in mediastinal diffuse large B cell lymphoma (DLBCL) and classical Hodgkin´s lymphoma. In B cell lymphomas of the gastrointestinal tract gains of chromosomal material in chromosomal region 2p13-p16 could also be detected using comparative genomic hybridization (CGH). Candidate genes for lymphomagenesis in this region are c-Rel and BCL11A.
The objective of this work was to shape out a potential gene dosis effect. For this purpose, 13 lymphomas of the gastrointestinal tract (8 diffuse large cell B cell lymphoma and 5 marginal zone B cell lymphoma) have been analyzed for their genomic status of c-Rel and BCL11A using fluorescence in situ hybridization (FISH). For 3 out of 13 lymphomas a gain of chromosomal material in chromosomal region 2p13-p16 had been detected. By using FISH a gain of gene number copies could be detected 5 lymphomas (3 of which had known gains of chromosomal material in CGH). The relative expression of c-Rel and BCL mRNA was detected using quantitative real time PCR. Furthermore, the expression and intracellular localization of the c-Rel and BCL11A protein were confirmed by using immunohistochemical staining with antibodies not commercially available. There was evidence for a stringen t gene dosis effect of c-Rel and BCL11A in only 2 out of 13 lymphomas. The remaining lymphomas present a heterogenous expression pattern. Maybe there are other regulatory mechanisms leading to constitutive activation of c-Rel and/ or BCL11A.
Date created
2009
Subject headings
[GND]: B-Zell-Lymphom[MeSH]: Carrier proteins | Genes, rel | Lymphoma, B-cell
[Free subject headings]: BCL11A | c-Rel | Gastrointestinal B cell lymphoma | Gene dosis effect
[DDC subject group]: DDC 610 / Medicine & health
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Please use this identifier to cite or link to this item: http://dx.doi.org/10.18725/OPARU-1593
Ehrlich, Steve (2009): Analyse des genomischen Status der Proto-Onkogene c-Rel und BCL11A sowie deren RNA- und Proteinexpression in Marginalzonen-B-Zell-Lymphomen und diffus großzelligen B-Zell-Lymphomen des Gastrointestinaltraktes. Open Access Repositorium der Universität Ulm und Technischen Hochschule Ulm. Dissertation. http://dx.doi.org/10.18725/OPARU-1593
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