Telomerlängenmessung von Nierenparenchymzellen und Neuronen des Rückenmarks zum Nachweis reduzierter Ischämie- und Reperfusionsschäden nach thorakalem Aortenclamping des Schweins bei präoperativer oraler Gabe von Superoxid-Dismutase (Glisodin®)
LicenseStandard (Fassung vom 01.10.2008)
Aortic crosclamping can lead to severe cell damage at kidneys and spinal cord, especially, as a result of ischemia and reperfusion (IR).The dimension of this damage can be determined by measuring the telomere-length (TL), which is also a standardized marker of senescence. The anti-oxidant enzyme Superoxid-Dismutase (SOD) is able to reduce cell damage. By means of an established pig model for IR it was tested, whether preoperative oral application of SOD would lead to significantly longer telomeres of kidney and spinal cord cells after 30 minutes of thoracic crossclamping and 4 hours of reperfusion. In total, 18 pigs of both gender were classified into one group with preoperative oral SOD-application and another group which has received maltodextrine as placebo. Furthermore, there was one additional group of nine kidneys deriving from non-operated pigs. The TL-measurement was carried out according to a defined protocol using gel electrophoresis, southern blot analysis and densitometric detection of chemoluminescence signals. As a result, there were no significantly different TL at all by comparison of all groups with each other. Approximately, the most probable reason for this was the fact, that -under the prevailing circumstances- a clamping period of 30 minutes was too short for a verifiable cell damage. TL-measurement will, nonetheless, play an important role in the evaluation of new therapy options for the reduction of cell damage caused by IR.
Subject HeadingsSuperoxiddismutasen [GND]
Telomer <Molekulargenetik> [GND]