Werner Syndrom Zellen haben eine veränderte transkriptionelle Regulation nach Wachstumsfaktorstimulation.
LicenseStandard (Fassung vom 03.05.2003)
Werner syndrome (WS) is a premature aging disease displaying defects in DNA replication, recombination, repair and transcription. WS is caused by mutations in a gene that encodes a RecQ DNA helicase (WRN). WRN is recruited into the nucleolus when rRNA transcription is reactivated in quiescent cells. We show that migration of WRN into the nucleolus can be facilitated through different single growth factors. This growth factors stimulate Pol I transcription in normal human fibroblasts although only 2 can activate Pol I transcription in WS cells, when WRN is lacking. WRN is known to interact with Pol I and we can show that the function of WRN in RNA polymerase I transcription is stimulation of transcription re-initiation. Further we show that WRN binds to the rDNA promoter and gen intern in quiescent cells. Transcription alterations in WS are striking similar to those in normal aging. We show that gene expression of WS cells is significantly disturbed after growth factor stimulation compared to normal cells.
Subject HeadingsProgeria adultorum [GND]
Transkription <Genetik> [GND]
Werner syndrome [MeSH]