Untersuchungen zur VEGF und PlGF induzierten Chemotaxis multipotenter Stromazellen des Knochenmarks
Leucht, Frank Martin
LicenseStandard (Fassung vom 03.05.2003)
Vascular endothelial growth factor (VEGF) has been indicated to play a role during endochondral ossification by stimulation of blood vessel invasion into hypertrophic cartilage resulting in its replacement by trabecular bone. We could demonstrate a dose-dependent chemoattractive effect of VEGF-A and PlGF-1 on human mesenchymal progenitor cells. Quantitative realtime PCR revealed the expression of VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4), which markedly declined during osteogenic differentiation. In addition, expression of neuropilin-1 and -2 was detected by RT-PCR. In an in vitro kinase assay, we could demonstrate activation of VEGFR-1 and VEGFR-2 upon stimulation with specific ligands. These findings are consistent with the idea that the chemotactic effect of VEGF-A on MPC is mediated via VEGFR-1 and that VEGF-A and PlGF-1 have a functional role for recruitment of osteoprogenitor cells in the course of endochondral bone formation or remodeling.
Original publicationBiochem Biophys Res Commun., Vol. 334, Iss. 2, 26 August 2005, pp. 561 - 568
Subject HeadingsChemotaxis [GND]
Vascular endothelial Growth Factor [GND]
Mesenchymal stem cells [MeSH]