• English
    • Deutsch
  • English 
    • English
    • Deutsch
  • Login
View Item 
  •   Home
  • Universität Ulm / Medizin
  • Publikationen
  • View Item
  •   Home
  • Universität Ulm / Medizin
  • Publikationen
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Einfluss einer Beatmung mit 100% Sauerstoff auf die Ausbildung von DNA-Strangbrüchen und die Parameter des oxidativen Stress im Langzeitmodell des septischen Schocks beim Schwein

Thumbnail
vts_6363_8560.pdf (1.623Mb)
109 Seiten
Veröffentlichung
2008-04-24
Authors
Gröger, Michael
Dissertation


Faculties
Medizinische Fakultät
Abstract
The letality of the septic shock did not decrease during the last decade and is still at about 50 %. Because of an increasing cost pressure there is a new focus on therapies, like ventilation with pure oxygen, which is easy and cheap to administer, but which also bears beside the positive effects potentially substantial risks: The stabilization of the circulation is opposed to the possible enhancement of the oxidative stress and the resulting organ, cell and DNA damage. Even though there was a lot of investigations on the effects of oxygen breathing on the formation of DNA damage in healthy organisms there is no knowledge about this in septic animals. In this study the genotoxic effects of different kinds of ventilation with pure oxygen were investigated in a hyperdynamic long time model of septic shock in swine. The peritonitis was induced by inoculation of autologous faeces in the abdomen. 12 and 24 hrs after the induction of sepsis DNA strand breaks were measured using the comet assay and the parameters of nitrosative and oxidative stress were analysed. At the end of the experiment the activity of the antioxidative enzymes SOD and catalase in lung and liver tissue were measured and a microbiological investigation of blood and ascites samples was done. To investigate the sensitivity of septic animals on an additional oxidative stress and to evaluate the capacity of DNA repair isolated lymphocytes were HBO exposed and monitored for two additional hours. The investigations showed that sepsis itself lead to a nitrosative and oxidative stress which leads to DNA strand breaks. Though, the hyperoxia did not lead to an additional increase in any of these parameters and also the antioxidative enzymes SOD and catalase did not show any difference. The ex vivo experiment with isolated lymphocytes sowed the same findings: However, HBO exposure lead to DNA damage but hyperoxia did not have any further influence on the amount of DNA strand breaks or on the DNA repair.
Date created
2007
Subject headings
[GND]: Comet Assay | DNS-Strangbruch | Oxidativer Stress | Septischer Schock
[MeSH]: Oxidative stress | Shock, septic | Swine
[Free subject headings]: DNA strand breaks | ESR
[DDC subject group]: DDC 610 / Medicine & health
License
Standard (Fassung vom 03.05.2003)
https://oparu.uni-ulm.de/xmlui/license_v1

Metadata
Show full item record

DOI & citation

Please use this identifier to cite or link to this item: http://dx.doi.org/10.18725/OPARU-1394

Gröger, Michael (2008): Einfluss einer Beatmung mit 100% Sauerstoff auf die Ausbildung von DNA-Strangbrüchen und die Parameter des oxidativen Stress im Langzeitmodell des septischen Schocks beim Schwein. Open Access Repositorium der Universität Ulm und Technischen Hochschule Ulm. Dissertation. http://dx.doi.org/10.18725/OPARU-1394
Citation formatter >



Policy | kiz service OPARU | Contact Us
Impressum | Privacy statement
 

 

Advanced Search

Browse

All of OPARUCommunities & CollectionsPersonsInstitutionsPublication typesUlm SerialsDewey Decimal ClassesEU projects UlmDFG projects UlmOther projects Ulm

My Account

LoginRegister

Statistics

View Usage Statistics

Policy | kiz service OPARU | Contact Us
Impressum | Privacy statement