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AuthorTyutyaev, Paveldc.contributor.author
Date of accession2018-09-03T08:27:13Zdc.date.accessioned
Available in OPARU since2018-09-03T08:27:13Zdc.date.available
Year of creation2017dc.date.created
Date of first publication2018-09-03dc.date.issued
AbstractCurrent through the σ-pore was first detected in hKv1.3_V388C channels, where the V388C mutation in hKv1.3 channels opened a new pathway (σ-pore) behind the central α-pore. Typical for this mutant channel was inward current at potentials more negative than -100 mV when the central α-pore was closed. The α-pore blockers such as TEA+ and peptide toxins (CTX, MTX) could not reduce current through the σ-pore of hKv1.3_V388C channels. This new pathway would proceed in parallel to the α-pore in the S6-S6 interface gap. To see whether this phenomenon is restricted to hKv1.3 channels we mutated hKv1.2 at the homologue position (hKv1.2_V370C). By overexpression of hKv1.2_V370C mutant channels in COS-7 cells we could show typical σ-currents. The electrophysiological properties of the σ-pore in hKv1.3_V388C and hKv1.2_V370C mutant channels were similar. The σ-pore of hKv1.2_V370C channels was most permeable to Na+ and Li+ whereas Cl- and protons did not influence current through the σ-pore. Tetraethylammonium (TEA+), charybdotoxin (CTX) and maurotoxin (MTX), known α-pore blockers, could not reduce current through the σ-pore of hKv1.2_V370C channels. Taken together we conclude that the observation of σ-pore currents is not restricted to Kv1.3 potassium channels but can also be observed in a closely related potassium channel. This finding could have implications in the treatment of different ion channel diseases linked to mutations of the respective channels in regions close to homologue position investigated by us.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandarddc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v3dc.rights.uri
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHIon channelsdc.subject.mesh
TitleUntersuchung der σ-Pore in spannungsabhängigen hKv-Kanälendc.title
Resource typeDissertationdc.type
Date of acceptance2018-06-29dcterms.dateAccepted
RefereeGrissmer, Stephandc.contributor.referee
RefereeFöhr, Karldc.contributor.referee
DOIhttp://dx.doi.org/10.18725/OPARU-9525dc.identifier.doi
PPN1030478252dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-oparu-9582-0dc.identifier.urn
GNDIonenkanaldc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
InstitutionInstitut für Angewandte Physiologieuulm.affiliationSpecific
Grantor of degreeMedizinische Fakultätuulm.thesisGrantor
DCMI TypeTextuulm.typeDCMI
CategoryPublikationenuulm.category


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