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AuthorHeckmann, Lesliedc.contributor.author
Date of accession2016-03-14T13:41:15Zdc.date.accessioned
Available in OPARU since2016-03-14T13:41:15Zdc.date.available
Year of creation2007dc.date.created
AbstractTendon and Ligament are essential part of the musculoskeletal system. Upon rupture of the cruciate ligaments, no spontaneous healing occurs. Therefore, the aim of this study was to evaluate the potential use of human Multipotent Mesenchymal Stroma Cells (MSC) and their differentiation towards a phenotype of Tenocytes (TC) in contact with different biomaterials for Tissue Engineering of tendon and ligament. A reference system consisting of a TC-seeded collagen type I-gel was characterized. The results of two culture systems with MSC were compared with the TC-model. MSC were cultured within the collagen gel and within a Poly-L-Lactide matrix (PLLA). Effects of TGFß-1, PDGF-bb and BMP13 were evaluated. Adhesion, proliferation and matrix metabolism were tested. Cell-matrix-interactions were characterized using Anti ß1-Integrin. Gene expression was evaluated with PCR techniques. Protein expression was determined by immuno histochemistry and zymography. Cells contracted the collagen matrices constantly during cultivation time. Contraction was abolished by addition of Anti-Integrin. Proliferation rates were between 60-80%. TC reduced collagen type I synthesis after introduction into the collagen gel, expression by MSC was slightly enhanced by 3D cultivation. Collagen type X was upregulated by TC and induced by MSC. On PLLA, expression of collagen type X was increased by TGFß-1. Increase in TenascinC expression was found in contracting collagen gels, not in the presence of Anti-Integrin or on PLLA. Integrin alpha-2 also was increased in collagen, but not on PLLA. Matrix degradation by MMPs was influenced by the biomaterial, too. MMP-1 was increased, MMP-13 induced in the collagen gel. Activation of MMP-2 was only found in collagen gel. The selection of the biomaterial and its adhesion conditions are critical parameters in Tissue Engineering influencing matrix synthesis and degradation. A modulating effect resulting from the presence of growth factors was detected.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandard (Fassung vom 03.05.2003)dc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v1dc.rights.uri
KeywordMSCdc.subject
KeywordPLLAdc.subject
KeywordTenozytendc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHStromal cellsdc.subject.mesh
MeSHTissue engineeringdc.subject.mesh
TitleGewebeersatz von Sehnen und Bändern: Untersuchungen zum Einfluss löslicher Faktoren und verschiedener Matrices auf humane multipotente mesenchymale Stromazellendc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-805dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-58918dc.identifier.urn
GNDKollagendc.subject.gnd
GNDSehnedc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
Date of activation2007-05-07T10:51:06Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionZ: J-H 11.450 ; W: W-H 9.575uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS ID5891uulm.vtsID
CategoryPublikationenuulm.category
Bibliographyuulmuulm.bibliographie


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