Show simple item record

AuthorWitton-Davies, Thomas Lyndc.contributor.author
Date of accession2016-03-14T13:41:13Zdc.date.accessioned
Available in OPARU since2016-03-14T13:41:13Zdc.date.available
Year of creation2006dc.date.created
AbstractThe comet assay (single-cell gel electrophoresis, SCG) is widely accepted as an in vitro and in vivo genotoxicity test. Because of its demonstrated ability to detect various kinds of DNA damage and its ease of application, the technique is being increasingly used in human biomonitoring. However, the assessment of small genotoxic effects as typically obtained in biomonitoring may be limited by the different sources of assay variability and the lack of an optimal protocol with high sensitivity. To better characterize the suitability of the comet assay for biomonitoring, we are performing a comprehensive investigation on blood samples from smokers and non-smokers. Because tobacco smoke is a well-documented source of a variety of potentially mutagenic and carcinogenic compounds, smokers should be a suitable study group with relevant mutagen exposure. Here, we report our results for the first sample of 20 healthy male smokers and 20 healthy male non-smokers. Baseline and benzo[a]pyrene diolepoxide (BPDE)-induced effects were analysed by two investigators using two image analysis systems. The study was repeated within 4 months. Furthermore, the influence of a repair inhibitor (aphidicolin, APC) on baseline and BPDE-induced DNA damage was comparatively analysed. In all experiments, a reference standard (untreated V79 cells) was included to correct for assay variability. None of these approaches revealed significant differences between smokers and non-smokers. Although more data is needed for a final conclusion, this study indicates some limitations of the comet assay with regard to the detection of DNA damage induced by environmental mutagens in peripheral blood cells.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandard (Fassung vom 03.05.2003)dc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v1dc.rights.uri
KeywordAphidicolindc.subject
KeywordBenzo[a]pyrendc.subject
Keywordbpdedc.subject
KeywordCytochrom P-450 1A1dc.subject
KeywordGlutathion-S-Transferasedc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
TitleUntersuchungen zum Einsatz des Comet Assays im Biomonitoringdc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-793dc.identifier.doi
PPN1651578001dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-58682dc.identifier.urn
GNDBiomonitoringdc.subject.gnd
GNDComet Assaydc.subject.gnd
GNDRauchendc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
Date of activation2007-03-09T08:37:51Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionZ: J-H 11.406 ; W: W-H 9.514uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS ID5868uulm.vtsID
CategoryPublikationenuulm.category
Bibliographyuulmuulm.bibliographie


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record