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AuthorSeizer, Peterdc.contributor.author
Date of accession2016-03-14T13:40:15Zdc.date.accessioned
Available in OPARU since2016-03-14T13:40:15Zdc.date.available
Year of creation2005dc.date.created
AbstractRepeated injections of hepatitis D antigen (HDAg) delivered either as a recombinant protein or expressed from a DNA vaccine elicited no (or only very low) antibody responses in inbred mouse strains. Codelivery of oligonucleotides (ODN) with immune-stimulating sequences (ISS) with the protein antigen or ISS in DNA vaccines (encoding HDAg) did not overcome the low intrinsic immunogenicity of this small viral antigen for B cells. In contrast, codelivery of immunogenic, heterologous proteins (either mixed to recombinant HDAg as recombinant proteins, or fused to HDAg sequences as chimeric antigens expressed from DNA vaccines) provided specific, CD4+ T cell-dependent "help" that supported efficient priming of antibody responses to HDAg. Chimeric proteins in which selected HDAg fragments were fused in frame with immunogenic, heterologous protein fragments produced by DNA vaccines allowed the mapping of antibody-binding HDAg domains of the viral antigen. The described approach thus facilitates induction of serum antibody responses against native viral antigens with low immunogenicity for B cells.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandard (Fassung vom 03.05.2003)dc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v1dc.rights.uri
KeywordCD4+ T-Zelledc.subject
KeywordDNA-Immunisierungdc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHAntibodiesdc.subject.mesh
MeSHAntigens, viraldc.subject.mesh
TitleCharakterisierung CD4+ T-Zell-vermittelter "Hilfe" für die Induktion von Antikörpern gegen schwach immunogene Antigenedc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-596dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-54544dc.identifier.urn
GNDAntikörperdc.subject.gnd
GNDHepatitis-D-Virusdc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
Date of activation2005-12-28T14:41:11Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionZ: J-H 10.986 ; W: W-H 8.933uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS ID5454uulm.vtsID
CategoryPublikationenuulm.category
Bibliographyuulmuulm.bibliographie


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