Improved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry

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peer-reviewed

Erstveröffentlichung
2022-06-30
Authors
Reich, Sebastian J.
Stohr, Jonas
Goldbeck, Oliver
Fendrich, Bastian
Crauwels, Peter
et al.
Wissenschaftlicher Artikel


Published in
MicrobiologyOpen ; 11 (2022), 4. - Art.-Nr. e1304. - ISSN 2045-8827
Link to original publication
https://dx.doi.org/10.1002/mbo3.1304
Faculties
Fakultät für Naturwissenschaften
Institutions
Institut für Mikrobiologie und Biotechnologie
Document version
published version (publisher's PDF)
Abstract
The global increase in antibiotic resistance of pathogenic microorganisms requires the identification and characterization of novel antimicrobials. Bacterial biosensors expressing fluorescent proteins such as pHluorin variants are suitable for high-throughput screenings. Here, we present Listeria spp. pH-sensitive biosensors with improved fluorescence for single-cell analysis of antimicrobials by flow cytometry.
Project uulm
AMPLIFY / Maßgeschneiderte Inhaltsstoffe 2 - Verbundvorhaben: "Pipeline und Zellfabriken zur Identifikation und Produktion neuer antimikrobieller Peptide (AMPLIFY) - Teilprojekt A" / BMBF / 031B0826A
Is supplemented by
https://www.ncbi.nlm.nih.gov/nuccore/ON668434
Subject headings
[GND]: Antimikrobieller Wirkstoff | Biosensor | Fluoreszenz
[LCSH]: Anti-infective agents | Biosensors | Fluorescence | Listeria
[Free subject headings]: antimicrobials | single‐cell analysis
[DDC subject group]: DDC 570 / Life sciences
License
CC BY 4.0 International
https://creativecommons.org/licenses/by/4.0/
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DOI & citation

Please use this identifier to cite or link to this item: http://dx.doi.org/10.18725/OPARU-48854

Reich, Sebastian J. et al. (2023): Improved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry. Open Access Repositorium der Universität Ulm und Technischen Hochschule Ulm. http://dx.doi.org/10.18725/OPARU-48854
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