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AuthorHofmann, Lindadc.contributor.author
AuthorLudwig, Sonjadc.contributor.author
AuthorSchuler, Patrick J.dc.contributor.author
AuthorHoffmann, Thomas K.dc.contributor.author
AuthorBrunner, Corneliadc.contributor.author
AuthorTheodoraki, Marie-Nicoledc.contributor.author
Date of accession2023-03-23T14:35:42Zdc.date.accessioned
Available in OPARU since2023-03-23T14:35:42Zdc.date.available
Date of first publication2020-05-26dc.date.issued
AbstractHead and neck squamous cell carcinomas (HNSCC) are highly immune suppressive and aggressive malignancies. As part of the tumor microenvironment, exosomes contribute to this immune suppression. The Fc receptor CD16 is widely expressed on monocytes, neutrophils, and natural killer (NK) cells and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC). Here, surface levels of CD16 on total exosomes and tumor-derived exosomes (TEX) from plasma of HNSCC patients were analyzed regarding their potential as liquid biomarkers for disease stage. Exosomes were isolated from plasma using mini size exclusion chromatography. TEX were enriched by immune affinity capture with CD44v3 antibodies. On-bead flow cytometry was used to measure CD16 levels on total exosomes and TEX. The results were correlated with clinicopathological parameters. Total exosomes from HNSCC patients had significantly higher CD16 levels compared to TEX. Further, CD16 surface levels of total exosomes, but not TEX, correlated with clinicopathological parameters. Patients with advanced tumor stages T3/4 and Union for International Cancer Control (UICC) stages III/IV had significantly higher CD16 levels on total exosomes compared to patients with early tumor stages T1/2 and UICC stages I/II, respectively. Overall, CD16 positive exosomes have the potential as liquid biomarkers for HNSCC tumor stage and aggressiveness.dc.description.abstract
Languageendc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseCC BY 4.0 Internationaldc.rights
Link to license texthttps://creativecommons.org/licenses/by/4.0/dc.rights.uri
KeywordHNSCCdc.subject
Keywordliquid biomarkerdc.subject
KeywordCD16dc.subject
Dewey Decimal GroupDDC 570 / Life sciencesdc.subject.ddc
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHExosomesdc.subject.mesh
MeSHSquamous Cell Carcinoma of Head and Neckdc.subject.mesh
MeSHReceptors, IgGdc.subject.mesh
TitleThe potential of CD16 on plasma-derived exosomes as a liquid biomarker in head and neck cancerdc.title
Resource typeWissenschaftlicher Artikeldc.type
SWORD Date2022-09-07T06:30:37Zdc.date.updated
VersionpublishedVersiondc.description.version
DOIhttp://dx.doi.org/10.18725/OPARU-47891dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-oparu-47967-9dc.identifier.urn
GNDBiomarkerdc.subject.gnd
InstitutionUKU. Klinik für Hals-, Nasen-, Ohrenheilkunde, Kopf- und Halschirurgieuulm.affiliationSpecific
Peer reviewjauulm.peerReview
DCMI TypeTextuulm.typeDCMI
CategoryPublikationenuulm.category
DOI of original publication10.3390/ijms21113739dc.relation1.doi
Source - Title of sourceInternational Journal of Molecular Sciencessource.title
Source - Place of publicationMDPIsource.publisher
Source - Volume21source.volume
Source - Issue11source.issue
Source - Year2020source.year
Source - Article number3739source.articleNumber
Source - eISSN1422-0067source.identifier.eissn
WoS000543400300001uulm.identifier.wos
Bibliographyuulmuulm.bibliographie


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