Oxidative stress response is mediated by overexpression and spatiotemporal regulation of caveolin-1
peer-reviewed
Erstveröffentlichung
2020-08-18Authors
Goutas, Andreas
Papathanasiou, Ioanna
Mourmoura, Evanthia
Tsesmelis, Konstantinos
Tsezou, Aspasia
Wissenschaftlicher Artikel
Published in
Antioxidants ; 9 (2020), 8. - Art.-Nr. 766. - eISSN 2076-3921
Link to original publication
https://dx.doi.org/10.3390/antiox9080766Faculties
Medizinische FakultätInstitutions
Institut für Physiologische ChemieDocument version
published version (publisher's PDF)Abstract
Oxidative stress (OS) has been linked to the aetiology of many diseases including osteoarthritis (OA). Recent studies have shown that caveolin-1—a structural protein of plasma membrane’s caveolae—is upregulated in response to OS. Here, we explore the function of caveolin-1 in chondrocytes derived from healthy individuals (control) and OA patients that were subjected to exogenous OS. We showed that caveolin-1 was upregulated in response to acute OS in the control, but not in OA chondrocytes. Moreover, OS-induced DNA damage analysis revealed that control cells started repairing the DNA lesions 6 h post-oxidative treatment, while OA cells seemed unable to restore these damages. Importantly, in the control cells, we observed a translocation of caveolin-1 from the membrane/cytoplasm in and out of the nucleus, which coincided with the appearance and restoration of DNA lesions. When caveolin-1 was prevented from translocating to the nucleus, the control cells were unable to repair DNA damage. In OA cells, no such translocation of caveolin-1 was observed, which could account for their inability to repair DNA damage. Taken together, these results provide novel insights considering the role of caveolin-1 in response to OS-induced DNA damage while revealing its implication in the pathophysiology of OA.
Subject headings
[GND]: Oxidativer Stress | Osteoarthritis | DNS-Schädigung | Altern | Mutagenität[MeSH]: Oxidative stress | Osteoarthritis | Cartilage, Articular | DNA damage | Caveolin 1 | Chondrocytes | Telomerase | Aging
[Free subject headings]: genotoxicity | senescence
[DDC subject group]: DDC 570 / Life sciences | DDC 610 / Medicine & health
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Please use this identifier to cite or link to this item: http://dx.doi.org/10.18725/OPARU-43549
Goutas, Andreas et al. (2022): Oxidative stress response is mediated by overexpression and spatiotemporal regulation of caveolin-1. Open Access Repositorium der Universität Ulm und Technischen Hochschule Ulm. http://dx.doi.org/10.18725/OPARU-43549
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