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AuthorFürst, Danieldc.contributor.author
AuthorTsamadou, Chrysanthidc.contributor.author
AuthorNeuchel, Christinedc.contributor.author
AuthorSchrezenmeier, Hubertdc.contributor.author
AuthorMytilineos, Joannisdc.contributor.author
AuthorWeinstock, Christofdc.contributor.author
Date of accession2022-06-27T13:21:20Zdc.date.accessioned
Available in OPARU since2022-06-27T13:21:20Zdc.date.available
Date of first publication2019-12-11dc.date.issued
AbstractSequencing of the human genome has led to the definition of the genes for most of the relevant blood group systems, and the polymorphisms responsible for most of the clinically relevant blood group antigens are characterized. Molecular blood group typing is used in situations where erythrocytes are not available or where serological testing was inconclusive or not possible due to the lack of antisera. Also, molecular testing may be more cost-effective in certain situations. Molecular typing approaches are mostly based on either PCR with specific primers, DNA hybridization, or DNA sequencing. Particularly the transition of sequencing techniques from Sanger-based sequencing to next-generation sequencing (NGS) technologies has led to exciting new possibilities in blood group genotyping. We describe briefly the currently available NGS platforms and their specifications, depict the genetic background of blood group polymorphisms, and discuss applications for NGS approaches in immunohematology. As an example, we delineate a protocol for large-scale donor blood group screening established and in use at our institution. Furthermore, we discuss technical challenges and limitations as well as the prospect for future developments, including long-read sequencing technologies. © In Copyright http://rightsstatements.org/vocab/InC/1.0/dc.description.abstract
Languageendc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseIn Copyrightdc.rights
Link to license texthttp://rightsstatements.org/vocab/InC/1.0/dc.rights.uri
KeywordNext-generation sequencingdc.subject
KeywordSequencingdc.subject
KeywordBlood groupdc.subject
KeywordDonor typing programdc.subject
KeywordRHDdc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHPhenotypedc.subject.mesh
MeSHDNA fingerprintingdc.subject.mesh
MeSHCellsdc.subject.mesh
MeSHGenomedc.subject.mesh
MeSHRecombination, Geneticdc.subject.mesh
TitleNext-generation sequencing technologies in blood group typingdc.title
Resource typeWissenschaftlicher Artikeldc.type
SWORD Date2021-02-04T13:28:16Zdc.date.updated
VersionpublishedVersiondc.description.version
DOIhttp://dx.doi.org/10.18725/OPARU-43532dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-oparu-43608-7dc.identifier.urn
GNDDNA-typingdc.subject.gnd
GNDGenomdc.subject.gnd
GNDPhänotypdc.subject.gnd
GNDBlutgruppedc.subject.gnd
GNDHLAdc.subject.gnd
InstitutionInstitut für Klinische Transfusionsmedizin und Immungenetik Ulm gGmbH (IKT)uulm.affiliationSpecific
InstitutionUKU. Institut für Transfusionsmedizinuulm.affiliationSpecific
Peer reviewjauulm.peerReview
DCMI TypeTextuulm.typeDCMI
CategoryPublikationenuulm.category
DOI of original publication10.1159/000504765dc.relation1.doi
Source - Title of sourceTransfusion Medicine and Hemotherapysource.title
Source - Place of publicationKarger Publisherssource.publisher
Source - Volume47source.volume
Source - Issue1source.issue
Source - Year2019source.year
Source - From page4source.fromPage
Source - To page13source.toPage
Source - ISSN1660-3796source.identifier.issn
Source - eISSN1660-3818source.identifier.eissn
CommunityWeitere Einrichtungenuulm.community
CommunityUniversitätsklinikum Ulmuulm.community
WoS000513158900002uulm.identifier.wos
PubMed32110189uulm.identifier.pubmed
Rights noticeDieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.dc.rights.other
Rights noticeThis publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.dc.rights.other
Bibliographyuulmuulm.bibliographie
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Is Supplemented Byhttps://www.karger.com/WebMaterial/ShowPic/1155418?imgType=1&file=img.jpgdc.relation.isSupplementedBy


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