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AuthorPars, Selindc.contributor.author
AuthorAchberger, Kevindc.contributor.author
AuthorKleger, Alexanderdc.contributor.author
AuthorLiebau, Stefandc.contributor.author
AuthorPashkovskaia, Nataliadc.contributor.author
Date of accession2022-06-23T13:51:25Zdc.date.accessioned
Available in OPARU since2022-06-23T13:51:25Zdc.date.available
Date of first publication2021-01-05dc.date.issued
AbstractHuman induced pluripotent stem cell (hiPSC)-derived endothelial cells (ECs) and pericytes provide a powerful tool for cardiovascular disease modelling, personalized drug testing, translational medicine, and tissue engineering. Here, we report a novel differentiation protocol that results in the fast and efficient production of ECs and pericytes from keratinocyte-derived hiPSCs. We found that the implementation of a 3D embryoid body (EB) stage significantly improves the differentiation efficiency. Compared with the monolayer-based technique, our protocol yields a distinct EC population with higher levels of EC marker expression such as CD31 and vascular endothelial cadherin (VE-cadherin). Furthermore, the EB-based protocol allows the generation of functional EC and pericyte populations that can promote blood vessel-like structure formation upon co-culturing. Moreover, we demonstrate that the EB-based ECs and pericytes can be successfully used in a microfluidic chip model, forming a stable 3D microvascular network. Overall, the described protocol can be used to efficiently differentiate both ECs and pericytes with distinct and high marker expression from keratinocyte-derived hiPSCs, providing a potent source material for future cardiovascular disease studies.dc.description.abstract
Languageendc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseCC BY 4.0 Internationaldc.rights
Link to license texthttps://creativecommons.org/licenses/by/4.0/dc.rights.uri
Keywordhuman pluripotent stem cellsdc.subject
KeywordhiPSC-derived endothelial cellsdc.subject
KeywordhiPSC-derived pericytesdc.subject
Keywordvasculature-on-a-chipdc.subject
Keywordself-assembled microvascular networkdc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHInduced pluripotent stem cellsdc.subject.mesh
MeSHEndothelial cellsdc.subject.mesh
TitleGeneration of functional vascular endothelial cells and pericytes from keratinocyte derived human induced pluripotent stem cellsdc.title
Resource typeWissenschaftlicher Artikeldc.type
SWORD Date2021-02-03T16:54:58Zdc.date.updated
VersionpublishedVersiondc.description.version
DOIhttp://dx.doi.org/10.18725/OPARU-43482dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-oparu-43558-0dc.identifier.urn
GNDInduzierte pluripotente Stammzelledc.subject.gnd
GNDEndothelzelledc.subject.gnd
InstitutionUKU. Klinik für Innere Medizin Iuulm.affiliationSpecific
Peer reviewjauulm.peerReview
DCMI TypeTextuulm.typeDCMI
CategoryPublikationenuulm.category
DOI of original publication10.3390/cells10010074dc.relation1.doi
Source - Title of sourceCellssource.title
Source - Place of publicationMDPIsource.publisher
Source - Volume10source.volume
Source - Issue1source.issue
Source - Year2021source.year
Source - Article number74source.articleNumber
Source - eISSN2073-4409source.identifier.eissn
Open AccessDOAJ Gold, Green Publisheduulm.OA
WoS000609995300001uulm.identifier.wos
Bibliographyuulmuulm.bibliographie
Is Supplemented Byhttps://www.mdpi.com/2073-4409/10/1/74/s1dc.relation.isSupplementedBy
Is Supplemented Byhttps://www.mdpi.com/2073-4409/10/1/74/htm#fig_body_display_cells-10-00074-f002dc.relation.isSupplementedBy


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