Single spins in diamond for nanoscale sensing and microscopy
FacultiesFakultät für Naturwissenschaften
InstitutionsInstitut für Quantenoptik
This thesis is dedicated to applications of nanodiamonds with colour centres for imaging and magnetic field sensing. Diamond nanocrystals with optically active defects such as nitrogen-vacancy (NV) or silicon-vacancy (SiV) can be used as optical markers. They are characterized by highly stable emission in contrast to other known optical markers. Owing to this they are attractive for studies of biological systems. First part of this thesis demonstrates application of nanodiamonds for drug delivery process. Stabilization of the nanocrystal’s surface in different solutions was achieved by coating with HSA-protein. Utilizing protein-protected nanodiamonds as carriers for chemotherapy drug (Doxorubicin), targeted delivery to the acidic tumor was demonstrated. Pre-clinical test proved the effectivity of this method compared to free drug molecules. The negatively charged nitrogen-vacancy centre due to unique combination of its optical and spin properties can be used as an atomic scale magnetic sensor. It combines in one high sensitivity and high resolution. In second part of this thesis these properties are exploited for detection of the metallo-protein – ferritin. The measurements of spin relaxation times allow to reveal the presence of ferritin in close vicinity of NV centre. The demonstrated experiments pave the way to a new method of ferritin’s core investigation, which is invaluable for diagnostics of different diseases.
Subject HeadingsDiamant [GND]
Biochemical markers [LCSH]
Drug delivery systems [LCSH]