A low‑cost open‑source 5‑choice operant box system optimized for electrophysiology and optophysiology in mice

peer-reviewed
Erstveröffentlichung
2021-11-15Autoren
Kapanaiah, Sampath K.T.
Van der Veen, Bastiaan
Strahnen, Daniel
Akam, Thomas
Kätzel, Dennis
Wissenschaftlicher Artikel
Erschienen in
Scientific Reports ; 11 (2021). - Art.-Nr. 22279. - eISSN 2045-2322
Link zur Originalveröffentlichung
https://dx.doi.org/10.1038/s41598-021-01717-1Fakultäten
Medizinische FakultätInstitutionen
Institut für Angewandte PhysiologieExterne Kooperationen
University of OxfordDokumentversion
Veröffentlichte Version (Verlags-PDF)Zusammenfassung
Operant boxes enable the application of complex behavioural paradigms to support circuit neuroscience and drug discovery research. However, commercial operant box systems are expensive and often not optimised for combining behaviour with neurophysiology. Here we introduce a fully open-source Python-based operant-box system in a 5-choice design (pyOS-5) that enables assessment of multiple cognitive and affective functions. It is optimized for fast turn-over between animals, and for testing of tethered mice for simultaneous physiological recordings or optogenetic manipulation. For reward delivery, we developed peristaltic and syringe pumps based on a stepper motor and 3D-printed parts. Tasks are specified using a Python-based syntax implemented on custom-designed printed circuit boards that are commercially available at low cost. We developed an open-source graphical user interface (GUI) and task definition scripts to conduct assays assessing operant learning, attention, impulsivity, working memory, or cognitive flexibility, alleviating the need for programming skills of the end user. All behavioural events are recorded with millisecond resolution, and TTL-outputs and -inputs allow straightforward integration with physiological recordings and closed-loop manipulations. This combination of features realizes a cost-effective, nose-poke-based operant box system that allows reliable circuit-neuroscience experiments investigating correlates of cognition and emotion in large cohorts of subjects.
DFG-Projekt uulm
Optogenetische und chemogenetische Analyse der kausalen Rolle der Hyperaktivität im ventralen Hippokampus für Symptome der Schizophrenie / DFG / 388492565 [KA 4594/2-1]
Publikationsförderung
Open-Access-Förderung durch die Medizinische Fakultät der Universität Ulm
Wird ergänzt durch
https://www.nature.com/articles/s41598-021-01717-1#Sec18Schlagwörter
[GND]: Neuropsychologie | Kognitionswissenschaft | Lernen | Gedächtnis[LCSH]: Cognitive neurosciences | Learning | Memory | Neural circuits
[DDC Sachgruppe]: DDC 610 / Medicine & health
Metadata
Zur LanganzeigeDOI & Zitiervorlage
Nutzen Sie bitte diesen Identifier für Zitate & Links: http://dx.doi.org/10.18725/OPARU-42063
Kapanaiah, Sampath K.T. et al. (2022): A low‑cost open‑source 5‑choice operant box system optimized for electrophysiology and optophysiology in mice. Open Access Repositorium der Universität Ulm und Technischen Hochschule Ulm. http://dx.doi.org/10.18725/OPARU-42063
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