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AuthorWu, Hangdc.contributor.author
Date of accession2022-01-27T13:09:45Zdc.date.accessioned
Available in OPARU since2022-01-27T13:09:45Zdc.date.available
Year of creation2020dc.date.created
Date of first publication2022-01-27dc.date.issued
AbstractThis study investigated the role of miRNAs in WAT tissue of mice either fed with normal diet (ND) or high fat diet (HFD). The expression of the miR-27a genes (miR-27a-5p and -3p) was up-regulated both in gonadal and inguinal WAT after HFD. In SGBS cell and human primary cell, the expression of miR-27a-5p and -3p was down-regulated upon adipogenic differentiation. This data suggests that the miR-27a-3p is potentially an important class of negative regulators of adipogenesis and may play a role in the regulation of adipose functions associated with obesity. This was confirmed by gain-of-function experiments, in which miR-27a-3p led to a robust and specific inhibition of adipogenic differentiation with the blockade of PPARγ, perilipin, and adiponectin expression, as well as reduced triglyceride and lipid droplets. miR-27a-5p did not show these effects. An in silico miRNA target prediction approach suggests that LPIN1 could be a possible target gene of miR-27a-3p and mediate the anti-adipogenic effects of this miRNA on adipogenic differentiation.dc.description.abstract
Languageen_USdc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseCC BY 4.0 Internationaldc.rights
Link to license texthttps://creativecommons.org/licenses/by/4.0/dc.rights.uri
KeywordMicroRNA-27adc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
LCSHFat cellsdc.subject.lcsh
MeSHMicroRNAsdc.subject.mesh
MeSHAdipocytesdc.subject.mesh
MeSHAdipogenesisdc.subject.mesh
MeSHModels, Animaldc.subject.mesh
MeSHMice, Obesedc.subject.mesh
TitleRole of microRNA-27a-5p and -3p in human adipocyte biologydc.title
Resource typeDissertationdc.type
Date of acceptance2021-11-11dcterms.dateAccepted
RefereeFischer-Posovsky, Pameladc.contributor.referee
RefereeKnippschild, Uwedc.contributor.referee
DOIhttp://dx.doi.org/10.18725/OPARU-41188dc.identifier.doi
PPN1787363147dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-oparu-41264-4dc.identifier.urn
GNDmiRNSdc.subject.gnd
GNDFettzelledc.subject.gnd
GNDTiermodelldc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
InstitutionUKU. Klinik für Kinder- und Jugendmedizinuulm.affiliationSpecific
InstitutionUKU. Klinik für Allgemein- und Viszeralchirurgieuulm.affiliationSpecific
Grantor of degreeMedizinische Fakultätuulm.thesisGrantor
DCMI TypeTextuulm.typeDCMI
CategoryPublikationenuulm.category
Bibliographyuulmuulm.bibliographie


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