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AuthorMartin, Inesdc.contributor.author
Date of accession2016-07-19T09:30:34Zdc.date.accessioned
Available in OPARU since2016-07-19T09:30:34Zdc.date.available
Year of creation2016dc.date.created
Date of first publication2016-07-19dc.date.issued
AbstractThe cytoskeleton, which consists of microtubules, actin filaments and intermediate filaments, stabilizes cells under mechanical stress. Especially important for stress resistance is the intermediate filament network. Since cells are very complex systems, it is difficult to obtain detailed knowledge about mechanics and functionality of single cytoskeletal components by only studying complete cells. Therefore, this thesis investigates intermediate filaments, the major contributor to cell rigidity, with the help of in vitro assembled keratin 8/18 networks. In particular the influence of monovalent ions on network stiffness is tested by using KCl and compared to crosslinking mediated by plectin. The results obtained with plectin in vitro are compared to extracted cells from the vulvar carcinoma derived cell line A431 with and without plectin downregulation. Furthermore, the special role of the C-terminal tail domain in network mechanics is examined. All samples are first analysed with electron microscopic methods to characterise the network architecture and subsequently mechanical properties are investigated with passive microrheology. Combining the findings from different methods and samples, a deeper insight in intermediate filament network mechanics is obtained. Setting these results in context with recent advances in other fields of biophysics and cell biology leads to a deeper knowledge of cell behaviour.dc.description.abstract
Languageendc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandarddc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v3dc.rights.uri
KeywordPlectindc.subject
KeywordPassive Microrheologydc.subject
Dewey Decimal GroupDDC 530 / Physicsdc.subject.ddc
LCSHKeratindc.subject.lcsh
LCSHElectron microscopydc.subject.lcsh
TitleArchitecture and mechanical properties of crosslinked Keratin 8/18 networksdc.title
Resource typeDissertationdc.type
Date of acceptance2016-06-08dcterms.dateAccepted
RefereeMarti, Othmardc.contributor.referee
RefereeGottschalk, Kay-Eberharddc.contributor.referee
DOIhttp://dx.doi.org/10.18725/OPARU-4047dc.identifier.doi
PPN864382189dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-oparu-4086-2dc.identifier.urn
GNDElektronenmikroskopiedc.subject.gnd
GNDMikrorheologiedc.subject.gnd
FacultyFakultät für Naturwissenschaftenuulm.affiliationGeneral
InstitutionInstitut für Experimentelle Physikuulm.affiliationSpecific
Shelfmark print versionW: W-H 14.802uulm.shelfmark
Grantor of degreeFakultät für Naturwissenschaftenuulm.thesisGrantor
DCMI TypeTextuulm.typeDCMI
TypeErstveröffentlichunguulm.veroeffentlichung
CategoryPublikationenuulm.category
FundingBaden-Württemberg Stiftung, Germany / Kompetenznetz Funktionelle Nanostrukturen (KFN) / Projekt A3uulm.funding
FundingBaden-Württemberg Stiftung, Germanyuulm.funding
University Bibliographyjauulm.unibibliographie


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