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Microbial utilization and bioconversion of lignocellulosic hydrolysates

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YanWang_Dissertation ... (29.14Mb)
Erstveröffentlichung
2021-12-09
Authors
Wang, Yan
Referee
Rosenau, Frank
Gottschalk, Kay-Eberhard
Dissertation


Faculties
Fakultät für Naturwissenschaften
Institutions
Institut für Pharmazeutische Biotechnologie
Institut für Experimentelle Physik
Abstract
Lignocellulosic biomass is the most abundant bio-resource on earth containing carbohydrates polymers cellulose, hemicellulose and lignin. With a number of methods including pretreatment and hydrolysis, lignocellulose-derived hydrolysates containing D-glucose, D-xylose, L-arabinose, and further sugars can be obtained. The hydrolysates consisting fermentable sugars typically display a high degree of variation depending on both the biomass source materials and process conditions, resulting in different compositions such as various concentrations of monosaccharide sugars and inhibitors. The fermentable sugars in lignocellulosic hydrolysates have attracted many people’s attention all over the world, because these fermentable sugars have a great potential to be used by microorganisms as a sole carbon source for the growth and production of bioproducts. To date, several bioproducts such as biofuels, chemicals and many high value-added products have been already investigated and produced, and the application field is still expanding. However, one of the key obstacles is that not all the microorganisms have the ability to metabolize all the different sugars in hydrolysates. Most of the known microorganisms only utilize D-glucose as the sole carbon source, which results in the energy loss of lignocellulosic biomass. Another obstacle is that the inhibitors formed during hydrolysis, limit the efficient usage of hydrolysates as a carbon source for biotechnological conversion. To further investigate and overcome these obstacles, in this Ph.D. thesis, the following research works have been examined and summarized: 1) Investigation the sugar utilization of Escherichia coli strains and the production of αs1-casein proteins based on lignocellulosic hydrolysates. 2) Metabolic engineering of Pseudomonas putida KT2440 as microbial biocatalyst for the utilization of D-xylose and L-arabinose. 3) Evaluation of different lignocellulosic hydrolysates as substrates for engineered P. putida KT2440 and the inhibitory effectiveness on P. putida KT2440.
Date created
2021
Cumulative dissertation containing articles
• Yan Wang, Dennis Kubiczek, Felix Horlamus, Heinz Fabian Raber, Till Hennecke, Daniel Einfalt, Marius Henkel, Rudolf Hausmann, Andreas Wittgens, Frank Rosenau. Bioconversion of lignocellulosic ‘waste’ to high value food proteins: recombinant production of bovine and human αS1-casein based on wheat straw lignocellulose. GCB Bioenergy.2021; 13:640-655. doi: 10.1111/gcbb.12791
• Felix Hoalamus, Yan Wang, David Steinbach, Maliheh Vahidinasab, Andreas Wittgens, Frank Rosenau, Marius Henkel, Rudolf Hausmann. Potential of biotechnological conversion of lignocellulose hydrolyzates by Pseudomonas putida KT2440 as a model organism for a bio-based economy. GCB Bioenergy.2019; 00:1-14. doi: 10.1111/gcbb.12647
• Yan Wang, Felix Horlamus, Marius Henkel, Filip Kovacic, Sandra Schläfle, Rudolf Hausmann, Andreas Wittgens, Frank Rosenau. Growth of engineered Pseudomonas putida KT2440 on glucose, xylose and arabinose: Hemicellulose hydrolysates and their major sugars as sustainable carbon sources. GCB Bioenergy.2019; 11: 249-259. doi: 10.1111/gcbb.12590
Subject headings
[GND]: Biomasse
[LCSH]: Biomass | Microbiology
[Free subject headings]: Lignocellulosic hydrolysates | Bioconversion | Metabolic engineering | Microbial biocatalyst
[DDC subject group]: DDC 540 / Chemistry & allied sciences
License
CC BY 4.0 International
https://creativecommons.org/licenses/by/4.0/

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DOI & citation

Please use this identifier to cite or link to this item: http://dx.doi.org/10.18725/OPARU-40294

Wang, Yan (2021): Microbial utilization and bioconversion of lignocellulosic hydrolysates. Open Access Repositorium der Universität Ulm und Technischen Hochschule Ulm. Dissertation. http://dx.doi.org/10.18725/OPARU-40294
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