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AuthorRetzmann, Denisedc.contributor.author
Date of accession2016-03-29T14:01:03Zdc.date.accessioned
Available in OPARU since2016-03-29T14:01:03Zdc.date.available
Year of creation2015dc.date.created
AbstractAims: Macrophage migration inhibitory factor (MIF) is an inflammatory cytokine widely expressed in vascular cells. Its proatherogenic role in lesion development has recently been detected in MIF-deficient mice. However, little is known how MIF regulates the contractile machinery of endothelial cells. Methods and results: Human umbilical vein endothelial cells (HUVECs) were stimulated with 40 ng/ml MIF for 30 min. Phosphorylation of mysion light chain (MLC) increased to 187±21 %, (n=5, p<0.05). At the same time cytoskeletal rearrangement and stress fibre formation occurred (immunohistochemical staining). Preincubation with the specific inhibitor of Src-Kinase PP2 (5 µmol/l) reduced MIF-induced MLC-phosphorylation to 81±7 % (n=5, p<0.05). Phosphorylation of Src following stimulation with MIF peaked after 15 minutes and reached 223±87 % (n=8, p<0.05). Also the downstream target of Src, PI-3-Kinase was activated as shown by kinase activity assay. PI-3-Kinase activity increased to 212±44 % compared to control (n=5, p<0.05). Concomitantly Akt phosphorylation increased to 173±12 % (n=4, p<0.05). Finally phosphorylation of ERK1/2 was analysed (209±16 %, n=5, p<0.05), indicating ERK activation after stimulation with MIF. Conclusions: We show here for the first time that MIF activates the contractile machinery of endothelial cells by phosphorylating MLC via Src-Kinase, PI3-Kinase/Akt and ERK1/2.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandarddc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v3dc.rights.uri
KeywordAtheriosclerosisdc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHArteriosclerosisdc.subject.mesh
MeSHMacrophage migration-inhibitory factorsdc.subject.mesh
TitleMacrophage migration inhibitory factor führt in humanen Endothelzellen zur Aktivierung der SRC-Kinase, der PI-3-Kinase und des MAP-Kinase Signalwegsdc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-3931dc.identifier.doi
PPN855864338dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-98839dc.identifier.urn
GNDArteriosklerosedc.subject.gnd
GNDMakrophagen-Inhibitionsfaktordc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
Date of activation2016-03-29T11:20:23Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionW: W-H 14.647uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS ID9883uulm.vtsID
CategoryPublikationenuulm.category
Bibliographyuulmuulm.bibliographie


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