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AuthorKrieg, Lauradc.contributor.author
AuthorSchaffert, Alexandradc.contributor.author
AuthorKern, Matthiasdc.contributor.author
AuthorLandgraf, Kathrindc.contributor.author
AuthorWabitsch, Martindc.contributor.author
AuthorBeck-Sickinger, Annette G.dc.contributor.author
AuthorKörner, Antjedc.contributor.author
AuthorBlüher, Matthiasdc.contributor.author
Authorvon Bergen, Martindc.contributor.author
AuthorSchubert, Kristindc.contributor.author
Date of accession2021-11-03T09:49:22Zdc.date.accessioned
Available in OPARU since2021-11-03T09:49:22Zdc.date.available
Date of first publication2020-02-11dc.date.issued
AbstractAdipokines and apolipoproteins are key regulators and potential biomarkers in obesity and associated diseases and their quantitative assessment is crucial for functional analyses to understand disease mechanisms. Compared to routinely used ELISAs, multiple reaction monitoring (MRM)-based mass spectrometry allows multiplexing and detection of proteins for which antibodies are not available. Thus, we established an MRM method to quantify 9 adipokines and 10 apolipoproteins in human serum. We optimized sample preparation by depleting the two most abundant serum proteins for improved detectability of low abundant proteins. Intra-day and inter-day imprecision were below 16.5%, demonstrating a high accuracy. In 50 serum samples from participants with either normal weight or obesity, we quantified 8 adipokines and 10 apolipoproteins. Significantly different abundances were observed for five adipokines (adipsin, adiponectin, chemerin, leptin, vaspin) and four apolipoproteins (apo-B100/-C2/-C4/-D) between the body mass index (BMI) groups. Additionally, we applied our MRM assay to serum samples from normal weight children and human adipocyte cell culture supernatants to proof the feasibility for large cohort studies and distinct biological matrices. In summary, this multiplexed assay facilitated the investigation of relationships between adipokines or apolipoproteins and phenotypes or clinical parameters in large cohorts, which may contribute to disease prediction approaches in the future.dc.description.abstract
Languageendc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseCC BY 4.0 Internationaldc.rights
Link to license texthttps://creativecommons.org/licenses/by/4.0/dc.rights.uri
KeywordMultiple reaction monitoringdc.subject
KeywordTargeted LC-MSdc.subject
KeywordHuman serumdc.subject
KeywordSGBS cellsdc.subject
KeywordCell culture supernatantdc.subject
Dewey Decimal GroupDDC 570 / Life sciencesdc.subject.ddc
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHAdipokinesdc.subject.mesh
MeSHApolipoproteinsdc.subject.mesh
MeSHChromatography, Liquiddc.subject.mesh
MeSHMass spectrometrydc.subject.mesh
MeSHObesitydc.subject.mesh
MeSHSerumdc.subject.mesh
TitleAn MRM-Based multiplexed quantification assay for human adipokines and apolipoproteinsdc.title
Resource typeWissenschaftlicher Artikeldc.type
SWORD Date2020-03-05T17:04:10Zdc.date.updated
VersionpublishedVersiondc.description.version
DOIhttp://dx.doi.org/10.18725/OPARU-39485dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-oparu-39561-8dc.identifier.urn
GNDAdipokinedc.subject.gnd
GNDApolipoproteinedc.subject.gnd
GNDLC-MSdc.subject.gnd
GNDFettsuchtdc.subject.gnd
GNDBlutserumdc.subject.gnd
InstitutionUKU. Klinik für Kinder- und Jugendmedizinuulm.affiliationSpecific
Peer reviewjauulm.peerReview
DCMI TypeTextuulm.typeDCMI
CategoryPublikationenuulm.category
In cooperation withHelmholtz-Zentrum Umweltforschung GmbH UFZuulm.cooperation
In cooperation withUniversität Leipziguulm.cooperation
DOI of original publication10.3390/molecules25040775dc.relation1.doi
Source - Title of sourceMoleculessource.title
Source - Place of publicationMDPIsource.publisher
Source - Volume25source.volume
Source - Issue4source.issue
Source - Year2020source.year
Source - Article number775source.articleNumber
Source - eISSN1420-3049source.identifier.eissn
Bibliographyuulmuulm.bibliographie


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