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AuthorHerrmann, Elenadc.contributor.author
AuthorYoung, Waynedc.contributor.author
AuthorReichert-Grimm, Verenadc.contributor.author
AuthorWeis, Severindc.contributor.author
AuthorRiedel, Christiandc.contributor.author
AuthorRosendale, Douglasdc.contributor.author
AuthorStoklosinski, Halinadc.contributor.author
AuthorHunt, Martindc.contributor.author
AuthorEgert, Markusdc.contributor.author
Date of accession2021-10-12T11:27:36Zdc.date.accessioned
Available in OPARU since2021-10-12T11:27:36Zdc.date.available
Date of first publication2018-02-06dc.date.issued
AbstractResistant starch (RS) is the digestion resistant fraction of complex polysaccharide starch. By reaching the large bowel, RS can function as a prebiotic carbohydrate, i.e., it can shape the structure and activity of bowel bacterial communities towards a profile that confers health benefits. However, knowledge about the fate of RS in complex intestinal communities and the microbial members involved in its degradation is limited. In this study, 16S ribosomal RNA (rRNA)-based stable isotope probing (RNA-SIP) was used to identify mouse bowel bacteria involved in the assimilation of RS or its derivatives directly in their natural gut habitat. Stable-isotope [U13C]-labeled native potato starch was administrated to mice, and caecal contents were collected before 0 h and 2 h and 4 h after administration. ‘Heavy’, isotope-labeled [13C]RNA species, presumably derived from bacteria that have metabolized the labeled starch, were separated from ‘light’, unlabeled [12C]RNA species by fractionation of isolated total RNA in isopycnic-density gradients. Inspection of different density gradients showed a continuous increase in ‘heavy’ 16S rRNA in caecal samples over the course of the experiment. Sequencing analyses of unlabeled and labeled 16S amplicons particularly suggested a group of unclassified Clostridiales, Dorea, and a few other taxa (Bacteroides, Turicibacter) to be most actively involved in starch assimilation in vivo. In addition, metabolic product analyses revealed that the predominant 13C-labeled short chain fatty acid (SCFA) in caecal contents produced from the [U13C] starch was butyrate. For the first time, this study provides insights into the metabolic transformation of RS by intestinal bacterial communities directly within a gut ecosystem, which will finally help to better understand its prebiotic potential and possible applications in human health.dc.description.abstract
Languageendc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseCC BY 4.0 Internationaldc.rights
Link to license texthttps://creativecommons.org/licenses/by/4.0/dc.rights.uri
Keywordgut microbiotadc.subject
KeywordRNA-SIPdc.subject
KeywordDoreadc.subject
Dewey Decimal GroupDDC 570 / Life sciencesdc.subject.ddc
MeSHResistant Starchdc.subject.mesh
MeSHGastrointestinal Microbiomedc.subject.mesh
MeSHClostridialesdc.subject.mesh
TitleIn vivo assessment of resistant starch degradation by the caecal microbiota of mice using RNA-based stable isotope probing—a proof-of-principle studydc.title
Resource typeWissenschaftlicher Artikeldc.type
SWORD Date2019-12-19T18:14:31Zdc.date.updated
VersionpublishedVersiondc.description.version
DOIhttp://dx.doi.org/10.18725/OPARU-38972dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-oparu-39048-9dc.identifier.urn
GNDMikrofloradc.subject.gnd
FacultyFakultät für Naturwissenschaftenuulm.affiliationGeneral
InstitutionInstitut für Mikrobiologie und Biotechnologieuulm.affiliationSpecific
Peer reviewjauulm.peerReview
DCMI TypeTextuulm.typeDCMI
CategoryPublikationenuulm.category
In cooperation withHochschule Furtwangenuulm.cooperation
In cooperation withAg Research Grasslands Research Centreuulm.cooperation
In cooperation withMassey Universityuulm.cooperation
In cooperation withUniversity of Aucklanduulm.cooperation
In cooperation withThe New Zealand Institute for Plant and Food Research Limiteduulm.cooperation
DOI of original publication10.3390/nu10020179dc.relation1.doi
Source - Title of sourceNutrientssource.title
Source - Place of publicationMDPIsource.publisher
Source - Volume10source.volume
Source - Issue2source.issue
Source - Year2018source.year
Source - Article number179source.articleNumber
Source - eISSN2072-6643source.identifier.eissn
FundingProject Präbio-SIP / Ministerium für Wissenschaft, Forschung und Kunst Baden-Württemberguulm.funding
FundingUniversität Furtwangenuulm.funding
Bibliographyuulmuulm.bibliographie


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