Evaluation of the gut microbiome in association with biological signatures of inflammation in murine polytrauma and shock
Appiah, Sandra A.
Foxx, Christine L.
Zambrano, Cristian A.
Scientific Reports ; 11 (2021). - Art.-Nr. 6665. - eISSN 2045-2322
Link to original publicationhttps://dx.doi.org/10.1038/s41598-021-85897-w
InstitutionsUKU. Klinik für Psychosomatische Medizin und Psychotherapie
UKU. Institut für Klinische und Experimentelle Trauma-Immunologie
UKU. Institut für Anästhesiologische Pathophysiologie und Verfahrensentwicklung
External cooperationsUniversity of Colorado Boulder
University of Colorado Anschutz Medical Campus
VA Eastern Colorado Health Care System
Military and Veteran Microbiome: Consortium for Research and Education
Document versionpublished version (publisher's PDF)
Severe injuries are frequently accompanied by hemorrhagic shock and harbor an increased risk for complications. Local or systemic inflammation after trauma/hemorrhage may lead to a leaky intestinal epithelial barrier and subsequent translocation of gut microbiota, potentially worsening outcomes. To evaluate the extent with which trauma affects the gut microbiota composition, we performed a post hoc analysis of a murine model of polytrauma and hemorrhage. Four hours after injury, organs and plasma samples were collected, and the diversity and composition of the cecal microbiome were evaluated using 16S rRNA gene sequencing. Although cecal microbial alpha diversity and microbial community composition were not found to be different between experimental groups, norepinephrine support in shock animals resulted in increased alpha diversity, as indicated by higher numbers of distinct microbial features. We observed that the concentrations of proinflammatory mediators in plasma and intestinal tissue were associated with measures of microbial alpha and beta diversity and the presence of specific microbial drivers of inflammation, suggesting that the composition of the gut microbiome at the time of trauma, or shortly after trauma exposure, may play an important role in determining physiological outcomes. In conclusion, we found associations between measures of gut microbial alpha and beta diversity and the severity of systemic and local gut inflammation. Furthermore, our data suggest that four hours following injury is too early for development of global changes in the alpha diversity or community composition of the intestinal microbiome. Future investigations with increased temporal-spatial resolution are needed in order to fully elucidate the effects of trauma and shock on the gut microbiome, biological signatures of inflammation, and proximal and distal outcomes.
DFG Project THU
SFB 1149 / Gefahrenantwort, Störfaktoren und regeneratives Potential nach akutem Trauma / DFG / 251293561
Open-Access-Förderung durch die Medizinische Fakultät der Universität Ulm
Is supplemented byhttps://www.nature.com/articles/s41598-021-85897-w#Sec19
Subject headings[GND]: Immunologie | Medizinische Mikrobiologie | Trauma | Polytrauma | Hämorrhagischer Schock
[MeSH]: Immunology | Microbiology | Shock, Hemorrhagic | Wounds and injuries | Microbiota
[DDC subject group]: DDC 610 / Medicine & health
LicenseCC BY 4.0 International
MetadataShow full item record
DOI & citation
Please use this identifier to cite or link to this item: http://dx.doi.org/10.18725/OPARU-38703