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AuthorNasr, Soaddc.contributor.author
AuthorRady, Maidc.contributor.author
AuthorSebak, Ayadc.contributor.author
AuthorGomaa, Imandc.contributor.author
AuthorFayad, Waliddc.contributor.author
AuthorElgaafary, Mennadc.contributor.author
AuthorAbdel-Kader, Mahmoud Hashemdc.contributor.author
AuthorSyrovets, Tatianadc.contributor.author
AuthorSimmet, Thomasdc.contributor.author
Date of accession2021-06-29T07:13:47Zdc.date.accessioned
Available in OPARU since2021-06-29T07:13:47Zdc.date.available
Date of first publication2020-05-29dc.date.issued
AbstractPhotodynamic therapy (PDT) is a non-invasive treatment strategy that includes the combination of three components—a photosensitizer, a light source, and tissue oxygen. PDT can be used for the treatment of skin diseases such as squamous cell carcinoma. The photosensitizer used in this study is the naturally derived chlorophyll derivative chlorin e6 (Ce6), which was encapsulated in ultradeformable ethosomes. Singlet oxygen production by Ce6 upon laser light irradiation was not significantly affected by encapsulation into ethosomes. PDT of squamous cell carcinoma cells treated with Ce6 ethosomes triggered increased mitochondrial superoxide levels and increased caspase 3/7 activity, resulting in concentration- and light-dose-dependent cytotoxicity. Ce6 ethosomes showed good penetration into 3D squamous cell carcinoma spheroids, which upon laser light irradiation exhibited reduced size, proliferation, and viability. The PDT effect of Ce6 ethosomes was specific and showed higher cytotoxicity against squamous cell carcinoma spheroids compared to normal skin fibroblast spheroids. In addition, PDT treatment of squamous cell carcinoma xenografts grown on chorioallantoic membranes of chick eggs (CAM) exhibited reduced expression of Ki-67 proliferation marker and increased terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining, indicating reduced proliferation and activation of apoptosis, respectively. The results demonstrate that Ce6-loaded ethosomes represent a convenient formulation for photodynamic treatment of squamous cell carcinoma.dc.description.abstract
Languageendc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseCC BY 4.0 Internationaldc.rights
Link to license texthttps://creativecommons.org/licenses/by/4.0/dc.rights.uri
Keyworddrug carrierdc.subject
Keywordchlorophyll derivativesdc.subject
Keywordphotodynamic therapydc.subject
Keywordsinglet oxygendc.subject
Keyword3D spheroid cell culturedc.subject
Keywordtumor xenograftdc.subject
Keywordchick chorioallantoic membrane assaydc.subject
Dewey Decimal GroupDDC 570 / Life sciencesdc.subject.ddc
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHCarcinoma, Squamous celldc.subject.mesh
MeSHCarcinoma, Squamous cell; Therapydc.subject.mesh
MeSHApoptosisdc.subject.mesh
MeSHReactive oxygen speciesdc.subject.mesh
TitleA naturally derived carrier for photodynamic treatment of squamous cell carcinoma: In vitro and In vivo modelsdc.title
Resource typeWissenschaftlicher Artikeldc.type
SWORD Date2020-12-09T19:40:16Zdc.date.updated
VersionpublishedVersiondc.description.version
DOIhttp://dx.doi.org/10.18725/OPARU-38085dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-oparu-38147-8dc.identifier.urn
GNDPlattenepithelcarcinomdc.subject.gnd
InstitutionUKU. Institut für Naturheilkunde und Klinische Pharmakologieuulm.affiliationSpecific
Peer reviewjauulm.peerReview
DCMI TypeTextuulm.typeDCMI
CategoryPublikationenuulm.category
In cooperation withThe American Iniversity in Cairouulm.cooperation
In cooperation withGerman University in Cairouulm.cooperation
In cooperation withOctober University for Modern Sciences & Arts - MSA Universityuulm.cooperation
In cooperation withNational Research Centeruulm.cooperation
In cooperation withCairo Univeristyuulm.cooperation
DOI of original publication10.3390/pharmaceutics12060494dc.relation1.doi
Source - Title of sourcePharmaceuticssource.title
Source - Place of publicationMDPIsource.publisher
Source - Volume12source.volume
Source - Issue6source.issue
Source - Year2020source.year
Source - Article number494source.articleNumber
Source - eISSN1999-4923source.identifier.eissn
Bibliographyuulmuulm.bibliographie
xmlui.metadata.uulm.OAfundingGefördert vom Ministerium für Wissenschaft, Forschung und Kunst Baden-Württemberguulm.OAfunding
xmlui.metadata.uulm.OAfundingOpen-Access-Förderung durch die Medizinische Fakultät der Universität Ulmuulm.OAfunding


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