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AutorGoerttler, Lukas Tsepodc.contributor.author
Aufnahmedatum2016-03-15T10:42:52Zdc.date.accessioned
In OPARU verfügbar seit2016-03-15T10:42:52Zdc.date.available
Jahr der Erstellung2015dc.date.created
ZusammenfassungAims: Chordoma is a tumour derived from chorda remnants which expresses early embryonal stem cell factors such as members of the sonic hedgehog pathway. We aimed at establishing new chordoma cell lines to define characteristic molecular patterns by gene expression profiling, thereby focusing on the sonic hedgehog pathway. Methods: Gene expression profiling was done by Agilent Human GE 4x44K Microarray. Selected targets were evaluated by PCR, Western blotting, immunocytology, and histology. Results: We established three new chordoma cell-lines (U-CH3, U-CH6, U-CH7, U-CH10) in addition to the published chordoma cell-lines U-CH1, U-CH2, and MUG-Chor1. The cell lines highly expressed brachyury, EMA, S100, and vimentin at protein and RNA levels. The seven cell lines clustered together when compared to 60 NCI cell lines (derived from carcinomas of breast, ovary, lung, kidney, prostate, and colon; melanoma, central nervous tumors, leukaemias). In all chordoma cell lines, sonic hedgehog (SHH) and smoothened (SMO) were highly expressed on RNA and protein levels. In addition, 30 chordomas showed a consistent expression of SHH and SMO in situ. Conclusion: The new chordoma cell lines serve as models for further analysis of this rare tumour entity and should foster research for successful treatment strategies. The sonic hedgehog reveals himself to be deficient.dc.description.abstract
Sprachededc.language.iso
Verbreitende StelleUniversität Ulmdc.publisher
LizenzStandarddc.rights
Link zum Lizenztexthttps://oparu.uni-ulm.de/xmlui/license_v3dc.rights.uri
SchlagwortAgilentdc.subject
SchlagwortChordomzellliniedc.subject
SchlagwortMicroarray gene expression profilingdc.subject
SchlagwortSonic Hedgehogdc.subject
DDC-SachgruppeDDC 610 / Medicine & healthdc.subject.ddc
MeSHBrachyury proteinsdc.subject.mesh
MeSHCell linedc.subject.mesh
MeSHChordomadc.subject.mesh
MeSHHedgehog proteinsdc.subject.mesh
MeSHMicroarray analysisdc.subject.mesh
MeSHNotochorddc.subject.mesh
MeSHSHH protein, humandc.subject.mesh
TitelEtablierung und Charakterisierung neuer Chordomzellliniendc.title
RessourcentypDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-3746dc.identifier.doi
PPN453410081dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-98265dc.identifier.urn
GNDChorda dorsalisdc.subject.gnd
GNDChordomdc.subject.gnd
GNDZellliniedc.subject.gnd
FakultätMedizinische Fakultätuulm.affiliationGeneral
Datum der Freischaltung2015-12-14T11:35:17Zuulm.freischaltungVTS
Peer-Reviewneinuulm.peerReview
Signatur DruckexemplarW: W-H 14.503uulm.shelfmark
DCMI MedientypTextuulm.typeDCMI
VTS-ID9826uulm.vtsID
KategoriePublikationenuulm.category


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