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AuthorElgaafary, Mennadc.contributor.author
AuthorHafner, Susannedc.contributor.author
AuthorLang, Sophia J.dc.contributor.author
AuthorJin, Ludc.contributor.author
AuthorSabry, Omar M.dc.contributor.author
AuthorVogel, Carl V.dc.contributor.author
AuthorVanderwal, Christopher D.dc.contributor.author
AuthorSyrovets, Tatianadc.contributor.author
AuthorSimmet, Thomasdc.contributor.author
Date of accession2021-03-29T13:37:49Zdc.date.accessioned
Available in OPARU since2021-03-29T13:37:49Zdc.date.available
Date of first publication2019-07-25dc.date.issued
AbstractBreast cancer is the most common cancer type and a primary cause of cancer mortality among females worldwide. Here, we analyzed the anticancer e cacy of a novel bromochlorinated monoterpene, PPM1, a synthetic analogue of polyhalogenated monoterpenes from Plocamium red algae and structurally similar non-brominated monoterpenes. PPM1, but not the non-brominated monoterpenes, decreased selectively the viability of several triple-negative as well as triple-positive breast cancer cells with di erent p53 status without significantly a ecting normal breast epithelial cells. PPM1 induced accumulation of triple-negative MDA-MB-231 cells with 4N DNA content characterized by decreased histone H3-S10/T3 phosphorylation indicating cell cycle arrest in the G2 phase. Western immunoblot analysis revealed that PPM1 treatment triggered an initial rapid activation of Aurora kinases A/B/C and p21Waf1/Cip1 accumulation, which was followed by accumulation of polyploid >4N cells. Flow cytometric analysis showed mitochondrial potential disruption, caspase 3/7 activation, phosphatidylserine externalization, reduction of the amount polyploid cells, and DNA fragmentation consistent with induction of apoptosis. Cell viability was partially restored by the pan-caspase inhibitor Z-VAD-FMK indicating caspase contribution. In vivo, PPM1 inhibited growth, proliferation, and induced apoptosis in MDA-MB-231 xenografted onto the chick chorioallantoic membrane. Hence, Plocamium polyhalogenated monoterpenes and synthetic analogues deserve further exploration as promising anticancer lead compounds.dc.description.abstract
Languageendc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseCC BY 4.0 Internationaldc.rights
Link to license texthttps://creativecommons.org/licenses/by/4.0/dc.rights.uri
Keywordred algaedc.subject
Keywordpolyhalogenated monoterpenesdc.subject
Keywordchick chorioallantoic membrane assaydc.subject
KeywordHALOGENATED MONOTERPENESdc.subject
KeywordHALOMONdc.subject
KeywordSENSITIVITYdc.subject
KeywordIDENTIFICATIONdc.subject
KeywordSTATISTICSdc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHBreast neoplasmsdc.subject.mesh
MeSHCell cycledc.subject.mesh
MeSHApoptosisdc.subject.mesh
MeSHRhodophytadc.subject.mesh
MeSHPlocamiumdc.subject.mesh
MeSHMonoterpenesdc.subject.mesh
MeSHOxidative stressdc.subject.mesh
TitleA novel polyhalogenated monoterpene induces cell cycle arrest and apoptosis in breast cancer cellsdc.title
Resource typeWissenschaftlicher Artikeldc.type
VersionpublishedVersiondc.description.version
DOIhttp://dx.doi.org/10.18725/OPARU-36419dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-oparu-36481-1dc.identifier.urn
GNDBrustkrebsdc.subject.gnd
GNDZellzyklusdc.subject.gnd
GNDMonoterpenedc.subject.gnd
GNDRotalgendc.subject.gnd
InstitutionUKU. Institut für Naturheilkunde und Klinische Pharmakologieuulm.affiliationSpecific
Peer reviewjauulm.peerReview
DCMI TypeTextuulm.typeDCMI
CategoryPublikationenuulm.category
In cooperation withCairo Universityuulm.cooperation
In cooperation withUniversity of Californiauulm.cooperation
DOI of original publication10.3390/md17080437dc.relation1.doi
Source - Title of sourceMarine Drugssource.title
Source - Place of publicationMDPIsource.publisher
Source - Volume17source.volume
Source - Issue8source.issue
Source - Year2019source.year
Source - Article number437source.articleNumber
Source - eISSN1660-3397source.identifier.eissn
FundingAcademic Center for Complementary and Integrative Medicineuulm.funding
FundingMinisterium für Wissenschaft, Forschung und Kunst Baden-Württemberguulm.funding
University Bibliographyjauulm.unibibliographie


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CC BY 4.0 International
Except where otherwise noted, this item's license is described as CC BY 4.0 International