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AuthorLeucht, Frank
AuthorBrenner, Rolf
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AbstractJoint injuries are highly associated with the development of post-traumatic osteoarthritis. Previous studies revealed cell- and matrix-protective e ects of N-acetylcysteine (NAC) after ex vivo cartilage trauma, while chondroanabolic stimulation with bone morphogenetic protein 7 (BMP7) enhanced type II collagen (COL2) expression. Here, as a next step, we investigated the combined and individual e cacy of intra-articular antioxidative and chondroanabolic treatment in a rabbit in vivo cartilage trauma model. Animals were randomly divided into group A (right joint: trauma (T); left joint: T+BMP7) and group B (right joint: T+NAC; left joint: T+BMP7+NAC). Condyles were impacted with the use of a spring-loaded impact device to ensure defined, single trauma administration. After 12 weeks, histopathological analysis was performed and the presence of matrix metalloproteinase 13 (MMP-13) and COL2 was assessed. Trauma-induced hypocellularity, MMP-13 expression, and cell cluster formation were reduced in NAC-treated animals. In contrast, BMP7 further increased cluster formation. Moreover, synovial concentrations of COL2 carboxy propeptide (CPII) and proteoglycan staining intensities were enhanced in NAC- and NAC+BMP7-treated joints. For the first time, the e cacy of NAC regarding early harm reduction after blunt cartilage trauma was demonstrated in vivo. However, parallel administration of BMP7 was not significantly superior compared to NAC alone.dc.description.abstract
PublisherUniversität Ulmdc.publisher
LicenseCC BY 4.0 Internationaldc.rights
Link to license text
KeywordPost-traumatic osteoarthritisdc.subject
KeywordBone morphogenic protein 7dc.subject
KeywordCartilage traumadc.subject
Dewey Decimal GroupDDC 540 / Chemistry & allied sciencesdc.subject.ddc
Dewey Decimal GroupDDC 570 / Life sciencesdc.subject.ddc
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHOsteoarthritis; Therapydc.subject.mesh
TitleInitial harm reduction by N-acetylcysteine alleviates cartilage degeneration after blunt single-impact cartilage trauma in vivodc.title
Resource typeWissenschaftlicher Artikeldc.type
InstitutionUKU. Klinik für Orthopädieuulm.affiliationSpecific
InstitutionBundeswehrkrankenhaus Ulm (BWK)uulm.affiliationSpecific
InstitutionUKU. Institut für Unfallchirurgische Forschung und Biomechanikuulm.affiliationSpecific
Peer reviewjauulm.peerReview
DCMI TypeTextuulm.typeDCMI
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DOI of original publication10.3390/ijms20122916dc.relation1.doi
Source - Title of sourceInternational Journal of Molecular Sciencessource.title
Source - Place of publicationMDPIsource.publisher
Source - Volume20source.volume
Source - Year2019source.year
Source - Article number2916source.articleNumber
Source - ISSN1661-6596source.identifier.issn
Source - eISSN1422-0067source.identifier.eissn
FundingBundesministerium der Verteidigung [E/U2A/CD524/DF560]uulm.funding
University Bibliographyjauulm.unibibliographie

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CC BY 4.0 International
Except where otherwise noted, this item's license is described as CC BY 4.0 International