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AuthorBartholomä, Jochendc.contributor.author
Date of accession2016-03-15T10:41:31Zdc.date.accessioned
Available in OPARU since2016-03-15T10:41:31Zdc.date.available
Year of creation2013dc.date.created
AbstractHexagonally arranged gold nanoparticles with controllable diameters and interparticle distances were deposited on thick SiO2 layers on top of Si wafers and used as masks during subsequent reactive ion etching. In this way, arrays of nanopillars are obtained with well-defined diameters (10/30 nm), inter-pillar distances (50 - 120 nm) and heights (20 - 50 nm), all on the nanoscale. Such nanotopographies served as substrate for mesenchymal stromal cells (MSC) and human osteoblasts (OB) allowing to study cellular responses to purely topographically patterned interfaces. Focus was put on adhesion, proliferation and differentiation of the cells. It turned out experimentally that adhesion is comparable for both cell types practically independent of topographical details at the substrate surface. Topography induced proliferation enhancement, however, is again independent of geometrical details in case of MSC, but significantly sensitive to pillar height in case of OB with a clear preference towards short nanopillars (20 nm). A high sensitivity to topographic details is also observed for osteogenic differentiation of MSC, in that case with a preference towards higher nanopillars (50 nm). The present experimental data also allow the important conclusion that cell proliferation and differentiation can be optimized simultaneously by fine-tuning nanoscaled topographical parameters.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandarddc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v3dc.rights.uri
KeywordMSZdc.subject
KeywordNanotopographiedc.subject
KeywordOBdc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHMesenchymal stromal cellsdc.subject.mesh
MeSHOsteoblastsdc.subject.mesh
TitleBeeinflussung multipotenter mesenchymaler Stromazellen und Osteoblasten durch nanostrukturierte Oberflächendc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-3421dc.identifier.doi
PPN1658722914dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-91151dc.identifier.urn
GNDMesenchymzelledc.subject.gnd
GNDOsteoblastdc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
Date of activation2014-07-28T14:07:16Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionW: W-H 13.735uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS ID9115uulm.vtsID
CategoryPublikationenuulm.category
Bibliographyuulmuulm.bibliographie


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