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AuthorMesserer, David Alexander Christiandc.contributor.author
AuthorVidoni, Lauradc.contributor.author
AuthorErber, Maikedc.contributor.author
AuthorStratmann, Alexander Elias Pauldc.contributor.author
AuthorBauer, Jonas Martindc.contributor.author
AuthorBraun, Christian Karldc.contributor.author
AuthorHug, Stefandc.contributor.author
AuthorAdler, Annadc.contributor.author
AuthorNilsson Ekdahl, Kristinadc.contributor.author
AuthorNilsson, Bodc.contributor.author
AuthorBarth, Eberharddc.contributor.author
AuthorRadermacher, Peterdc.contributor.author
AuthorHuber-Lang, Markusdc.contributor.author
Date of accession2020-12-10T13:37:20Zdc.date.accessioned
Available in OPARU since2020-12-10T13:37:20Zdc.date.available
Date of first publication2020-10-14dc.date.issued
ISSN1664-3224dc.identifier.issn
AbstractStudying innate immunity in humans is crucial for understanding its role in the pathophysiology of systemic inflammation, particularly in the complex setting of sepsis. Therefore, we standardized a step-by-step process from the venipuncture to the transfer in a human model system, while closely monitoring the inflammatory response for up to three hours. We designed an animal-free, human whole blood sepsis model using a commercially available, simple to use, tubing system. First, we analyzed routine clinical parameters, including cell count and blood gas analysis. Second, we demonstrated that extracellular activation markers (e.g., CD11b and CD62l) as well as intracellular metabolic (intracellular pH) and functional (generation of radical oxygen species) features remained stable after incubation in the whole blood model. Third, we mimicked systemic inflammation during early sepsis by exposure of whole blood to pathogen-associated molecular patterns. Stimulation with lipopolysaccharide revealed the capability of the model system to evoke a sepsis-like inflammatory phenotype of innate immunity. In summary, the presented model serves as a convenient, economic, and reliable platform to study innate immunity in human whole blood, which may yield clinically important insights.dc.description.abstract
Languageendc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseCC BY 4.0 Internationaldc.rights
Link to license texthttps://creativecommons.org/licenses/by/4.0/dc.rights.uri
Keywordneutrophil granulocytesdc.subject
Keywordblood physiologydc.subject
Keywordex vivo whole blood modeldc.subject
Keywordprinciples of the 3Rsdc.subject
Keywordneutrophil dysfunctiondc.subject
Keywordoxidative burstdc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHInflammationdc.subject.mesh
MeSHGranulocytesdc.subject.mesh
MeSHBlood physiological phenomenadc.subject.mesh
MeSHNeutrophilsdc.subject.mesh
MeSHShock, Septicdc.subject.mesh
MeSHPhagocytosisdc.subject.mesh
MeSHReceptor, Anaphylatoxin C5adc.subject.mesh
MeSHLipopolysaccharidesdc.subject.mesh
MeSHSepsisdc.subject.mesh
TitleAnimal-Free human whole blood sepsis model to study changes in innate immunitydc.title
Resource typeWissenschaftlicher Artikeldc.type
VersionpublishedVersiondc.description.version
DOIhttp://dx.doi.org/10.18725/OPARU-34046dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-oparu-34108-8dc.identifier.urn
GNDNeutrophiler Granulozytdc.subject.gnd
GNDEntzündungdc.subject.gnd
GNDSepsisdc.subject.gnd
InstitutionUKU. Institut für Klinische und Experimentelle Trauma-Immunologieuulm.affiliationSpecific
InstitutionUKU. Klinik für Anästhesiologieuulm.affiliationSpecific
InstitutionUKU. Institut für Anästhesiologische Pathophysiologie und Verfahrensentwicklunguulm.affiliationSpecific
Peer reviewjauulm.peerReview
DCMI TypeTextuulm.typeDCMI
CategoryPublikationenuulm.category
In cooperation withRudbeck Laboratory Uppsalauulm.cooperation
In cooperation withLinnaeus Universityuulm.cooperation
DOI of original publication10.3389/fimmu.2020.571992dc.relation1.doi
Source - Title of sourceFrontiers in Immunologysource.title
Source - Place of publicationFrontiers Mediasource.publisher
Source - Volume11source.volume
Source - Year2020source.year
Source - Article number571992source.articleNumber
Source - eISSN1664-3224source.identifier.eissn
Open AccessDOAJ Gold, Green Publisheduulm.OA
CommunityUniversitätsklinikum Ulmuulm.community
Bibliographyuulmuulm.bibliographie
DFG project uulmSFB 1149 / Gefahrenantwort, Störfaktoren und regeneratives Potential nach akutem Trauma / DFG / 251293561uulm.projectDFG
xmlui.metadata.uulm.OAfundingOpen-Access-Förderung durch die Medizinische Fakultät der Universität Ulmuulm.OAfunding


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