Animal-Free human whole blood sepsis model to study changes in innate immunity
peer-reviewed
Erstveröffentlichung
2020-10-14Authors
Messerer, David Alexander Christian
Vidoni, Laura
Erber, Maike
Stratmann, Alexander Elias Paul
Bauer, Jonas Martin
Wissenschaftlicher Artikel
Published in
Frontiers in Immunology ; 11 (2020). - Art.-Nr. 571992. - eISSN 1664-3224
Link to original publication
https://dx.doi.org/10.3389/fimmu.2020.571992Institutions
UKU. Institut für Klinische und Experimentelle Trauma-ImmunologieUKU. Klinik für Anästhesiologie
UKU. Institut für Anästhesiologische Pathophysiologie und Verfahrensentwicklung
External cooperations
Rudbeck Laboratory UppsalaLinnaeus University
ISSN
1664-3224
Document version
published version (publisher's PDF)Abstract
Studying innate immunity in humans is crucial for understanding its role in the
pathophysiology of systemic inflammation, particularly in the complex setting of sepsis.
Therefore, we standardized a step-by-step process from the venipuncture to the transfer
in a human model system, while closely monitoring the inflammatory response for up to
three hours. We designed an animal-free, human whole blood sepsis model using a
commercially available, simple to use, tubing system. First, we analyzed routine clinical
parameters, including cell count and blood gas analysis. Second, we demonstrated that
extracellular activation markers (e.g., CD11b and CD62l) as well as intracellular metabolic
(intracellular pH) and functional (generation of radical oxygen species) features remained
stable after incubation in the whole blood model. Third, we mimicked systemic
inflammation during early sepsis by exposure of whole blood to pathogen-associated
molecular patterns. Stimulation with lipopolysaccharide revealed the capability of the
model system to evoke a sepsis-like inflammatory phenotype of innate immunity.
In summary, the presented model serves as a convenient, economic, and reliable
platform to study innate immunity in human whole blood, which may yield clinically
important insights.
DFG Project THU
SFB 1149 / Gefahrenantwort, Störfaktoren und regeneratives Potential nach akutem Trauma / DFG / 251293561
Publication funding
Open-Access-Förderung durch die Medizinische Fakultät der Universität Ulm
Subject headings
[GND]: Neutrophiler Granulozyt | Entzündung | Sepsis[MeSH]: Inflammation | Granulocytes | Blood physiological phenomena | Neutrophils | Shock, Septic | Phagocytosis | Receptor, Anaphylatoxin C5a | Lipopolysaccharides | Sepsis
[Free subject headings]: neutrophil granulocytes | blood physiology | ex vivo whole blood model | principles of the 3Rs | neutrophil dysfunction | oxidative burst
[DDC subject group]: DDC 610 / Medicine & health
Metadata
Show full item recordDOI & citation
Please use this identifier to cite or link to this item: http://dx.doi.org/10.18725/OPARU-34046
Messerer, David Alexander Christian et al. (2020): Animal-Free human whole blood sepsis model to study changes in innate immunity. Open Access Repositorium der Universität Ulm und Technischen Hochschule Ulm. http://dx.doi.org/10.18725/OPARU-34046
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