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AuthorMahaddalkar, Pallavidc.contributor.author
Date of accession2016-03-15T10:40:35Zdc.date.accessioned
Available in OPARU since2016-03-15T10:40:35Zdc.date.available
Year of creation2014dc.date.created
AbstractRecently, various studies have tried to address this impounding question by reprogramming various cell populations. It had been successfully shown that stem/progenitor cells derived from different organs reprogram more efficiently than their differentiated counterparts thus unveiling a hierarchical reprogramming efficiency in the cells from different organs. Nevertheless, the molecular mechanisms governing this graded reprogramming phenomenon were yet to be explored. To analyse the molecular players behind this grading in reprogramming, we aimed to investigate the reprogramming capacity of different types of cells from liver parenchyma, an endodermal derived organ. In our study we found that different types of liver cells exhibit a kind of hierarchy during reprogramming towards induced pluripotent stem cells. More precisely, liver progenitor cells (LPCs) showed 275-fold superiority in reprogramming compared to differentiated liver cells (non-LPCs). When subjected to various molecular tests, LPCs endogenously express certain reprogramming factors but omission of those factors still did not allow successful reprogramming. On the other hand, LPCs showed higher reprogramming efficiencies with similar proliferation rates as their differentiated equivalents thus ruling out the effect of proliferation but impressing on the association of expression of endogenous pluripotency factors on higher reprogramming efficiencies. The presence of BAF (Brg1/Brm associated factor)-complex members Baf155 and Brg1 appeared to mediate the superior reprogramming in LPCs compared to non-LPCs as the knockdown of these BAF complex members annulled the increase reprogramming efficiencies of LPCs compared to non-LPCs.dc.description.abstract
Languageendc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandarddc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v3dc.rights.uri
KeywordBAF complexdc.subject
KeywordMouse liverdc.subject
KeywordReprogrammingdc.subject
Dewey Decimal GroupDDC 570 / Life sciencesdc.subject.ddc
MeSHPluripotent stem cellsdc.subject.mesh
TitleReprogramming hierarchy in mouse liver cell subpopulations depends on cell intrinsic factors including BAF complex members.dc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-3302dc.identifier.doi
PPN806913185dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-92453dc.identifier.urn
GNDVorläuferzelledc.subject.gnd
FacultyFakultät für Naturwissenschaftenuulm.affiliationGeneral
Date of activation2014-11-21T08:40:29Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionW: W-H 13.876uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS-ID9245uulm.vtsID
CategoryPublikationenuulm.category
University Bibliographyjauulm.unibibliographie


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