Show simple item record

AuthorSteinacker, Jochendc.contributor.author
Date of accession2016-03-15T09:09:27Zdc.date.accessioned
Available in OPARU since2016-03-15T09:09:27Zdc.date.available
Year of creation2012dc.date.created
AbstractThe species Leishmania major is an important parasite which causes diseases named leishmaniasis in humans. Leishmania has been in the focus of scientific studies in the last decade, in which a crucial finding was the detection of morphological characteristics of apoptosis in unicellular parasites. In contrast, molecular pathways of apoptosis in protozoa remained unidentified to a large extent. Therefore, our first attempt was to further explore several known processes during apoptosis in Leishmania major. In stimulation studies amastigotes and promastigotes showed different susceptibility against chemicals, the first became more positive for annexin V staining after staurosporine stimulation, the latter developed a larger subpopulation of dead cells after stimulation with miltefosine. Analysis with fluorescence-activated cell sorting (FACS) confirmed the apoptotic potential for both staurosporine and miltefosine by showing subpopulations of hypoploid cells after stimulation. In the next step, the role of reactive oxygen species (ROS) during apoptosis induction was elicited with FACS analysis. Data showed stage-specific differences with promastigotes being physiological negative for ROS and amastigotes being highly positive for ROS staining. The postulated influence of ROS during apoptosis mediation was supported by promastigotes becoming highly positive for ROS after apoptotic stimulation. Application of ROS scavengers led to a decline of the hypoploid subpopulation. The following experiments focused on the measurement of enzyme regulation of Leishmania major ascorbate Peroxidase (LmAPX) and Endonuclease G (EndoG), data obtained from light cycler and fluorescence microscopy probes strengthened the hypothesis of LmAPX being an antiapoptotic and EndoG being a pro-apoptotic enzyme. New possible mediators of apoptosis were identified after blocking protein synthesis with cycloheximide, a calpain-like cystein peptidase might have anti-apoptotic effects in Leishmania.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandarddc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v3dc.rights.uri
KeywordEndonuclease Gdc.subject
KeywordLeishmania major ascorbate peroxidasedc.subject
KeywordProgrammed cell deathdc.subject
KeywordPropidium iodide stainingdc.subject
KeywordProtozoadc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHApoptosisdc.subject.mesh
MeSHLeishmaniadc.subject.mesh
MeSHReactive oxygen speciesdc.subject.mesh
TitleDetektion und Analyse molekularer Mechanismen der Apoptose in humanpathogenen Protozoen der Gattung Leishmaniadc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-3113dc.identifier.doi
PPN787552054dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-89877dc.identifier.urn
GNDProtozoendc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
Date of activation2014-05-28T11:36:52Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionW: W-H 13.642uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS-ID8987uulm.vtsID
CategoryPublikationenuulm.category
University Bibliographyjauulm.unibibliographie


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record