Zur Kurzanzeige

AutorHärtel, Barbaradc.contributor.author
Aufnahmedatum2016-03-15T09:04:56Zdc.date.accessioned
In OPARU verfügbar seit2016-03-15T09:04:56Zdc.date.available
Jahr der Erstellung2013dc.date.created
ZusammenfassungMEF and MORF proteins are two groups of RNA editing trans factors. In this thesis representatives of both groups were further charaterized. In two different approaches - via EMS mutant lines and via T-DNA insertion lines of so far uncharacterized PPR proteins - five new PPR proteins could be identified as required for RNA editing at one to six sites in mitochondria of Arabidopsis thaliana. MEF10 is needed for editing of a site in nad2, MEF12 of a site in nad5, MEF13 of six sites in transcripts of ccmFc, cox3, nad2, nad4, nad5 and nad7. MEF13 transgenically expressed in mef13-1 plants additionally increases editing at another site which is only weakly edited in wildtype plants. MEF28 is responsible for editing of one of two or both neighbouring sites in the nad2 transcript. MEF30 is required for editing of two sites in nad4 und cyt. In mutants of the two chloroplast targeted MORF proteins, MORF2 and MORF9, all chloroplast editing sites were examined. Both proteins are needed for editing of almost all plastidic sites. First, the intracellular localization of all nine MORF proteins was examined by fluorescence microscopy to further characterize both groups of RNA editing actors. In BiFC assays in tobacco epidermal cells interactions between MORF proteins as well as between MORF and PPR proteins were shown. Furthermore, editing mutants with defects in editing at two sites in the matR transcript were used to elucidate the potenial function of matR as a maturase.dc.description.abstract
Sprachededc.language.iso
Verbreitende StelleUniversität Ulmdc.publisher
LizenzStandarddc.rights
Link zum Lizenztexthttps://oparu.uni-ulm.de/xmlui/license_v3dc.rights.uri
SchlagwortMEFdc.subject
SchlagwortMORFdc.subject
DDC-SachgruppeDDC 570 / Life sciencesdc.subject.ddc
LCSHArabidopsisdc.subject.lcsh
LCSHMitochondriadc.subject.lcsh
LCSHRNA editingdc.subject.lcsh
LCSHTranscription factorsdc.subject.lcsh
MeSHTranscription factorsdc.subject.mesh
TitelMEFs und MORFs: Charakterisierung von Vertretern zweier Arten von RNA-Editing-trans-Faktorendc.title
RessourcentypDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-2607dc.identifier.doi
PPN380846721dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-84342dc.identifier.urn
GNDMitochondriumdc.subject.gnd
GNDRNS-Edierungdc.subject.gnd
FakultätFakultät für Naturwissenschaftenuulm.affiliationGeneral
Datum der Freischaltung2013-04-05T11:46:24Zuulm.freischaltungVTS
Peer-Reviewneinuulm.peerReview
Signatur DruckexemplarW: W-H 13.236uulm.shelfmark
DCMI MedientypTextuulm.typeDCMI
VTS-ID8434uulm.vtsID
KategoriePublikationenuulm.category


Dateien zu dieser Ressource

Thumbnail

Das Dokument erscheint in:

Zur Kurzanzeige