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AuthorHagemann, Jürgen Benjamindc.contributor.author
Date of accession2016-03-15T06:25:23Zdc.date.accessioned
Available in OPARU since2016-03-15T06:25:23Zdc.date.available
Year of creation2011dc.date.created
AbstractThe main issue of this experimental work was to investigate the regulation of antimicrobial peptides in tuberculosis with a special focus on T-cell clones (characterized as CD4+, TZR alpha ß+, CD1-restricted and specifically activated by mycobacterial lipid antigens), macrophages (M Phi) and peripheral blood mononuclear cells (PBMC). The expression of a selective panel of immunologically important molecules was analyzed using quantitative real time PCR (qRT-PCR), the enzyme linked immunosorbent assay (ELISA) as well as flow cytometry. After stimulation with mycobacterial lipid antigens, we measured the regulation of cathepsin G (CathG), cathelicidin, toll-like receptor 2 (TLR-2) and purinoceptor P2X7 in M Phi on the one hand as well as the expression of gamma-interferon (IFN gamma), perforin and granulysin in T-cell clones on the other hand. The results found were then transferred to primary human cells (monocytes and T-lymphocytes) generated from PBMC. We found that stimulation with mycobacterial antigens leads to a significant up-regulation of TLR-2 and P2X7 in M Phi as well as to an increase of the transcription and secretion of IFN gamma in T-cell clones. The latter also expressed more granulysin - a result likewise reproducible in primary human T-lymphocytes of PPD+ donors, especially in the CD4+ subpopulation. Our data therefore support a theory assuming mycobacterial lipids to have an enormous potential as regards manipulating and inducing protective immunity. Selective application of mycobacterial lipid antigens in the context of preventive strategies and vaccination might stimulate protective T-cell populations expressing granulysin, enabling them to avoid active tuberculosis. The data collected suggest using lipids as possible vaccination antigens or as immunomodulatory molecules in a targeted therapy of tuberculosis.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandarddc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v3dc.rights.uri
KeywordGranulysindc.subject
KeywordLipidantigenedc.subject
KeywordP2X7dc.subject
KeywordPBMCdc.subject
KeywordqRT-PCRdc.subject
KeywordTLR-2dc.subject
KeywordTuberkulosedc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHEnzyme linked immunosorbent assaydc.subject.mesh
MeSHTuberculosisdc.subject.mesh
TitleRegulation von antimikrobiellen Peptiden bei der Tuberkulosedc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-2328dc.identifier.doi
PPN715609505dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-79058dc.identifier.urn
GNDAntigen CD4dc.subject.gnd
GNDEnzyme-linked immunosorbent assaydc.subject.gnd
GNDT-Lymphozytdc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
Date of activation2012-04-24T09:59:33Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionW: W-H 12.851uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS-ID7905uulm.vtsID
CategoryPublikationenuulm.category


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