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AuthorXiao, Zhengdc.contributor.author
Date of accession2016-03-14T11:55:03Zdc.date.accessioned
Available in OPARU since2016-03-14T11:55:03Zdc.date.available
Year of creation2002dc.date.created
AbstractA single cell in vivo green fluorescence assay was successfully developed to monitor effectively V(D)J recombination efficiency. Our results show that the in vivo V(D)J recombination efficiency is essentially low (ca. 1 %) in 293 cells even though the transfection efficiency reached 60 % to 90 %. This in vivo V(D)J recombination efficiency correlated well with that achieved by the classical in vitro V(D)J recombination assay. The in vivo V(D)J recombination efficiency depended on the relative RAG-1 and RAG-2 expression vector concentrations used in co-transfection, but was independent of relative Artemis expression vector concentrations. The novel in vivo EGFP V(D)J recombination assay has been successfully set up and can in the future be applied for testing the efficiency of V(D)J recombination in cell lines, for evaluating roles of components in V(D)J recombination, for searching for novel factors involved in the V(D)J recombination process and for diagnosing SCID patients.dc.description.abstract
Languageendc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandard (Fassung vom 03.05.2003)dc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v1dc.rights.uri
KeywordEGFPdc.subject
KeywordV(D)Jdc.subject
MeSHSevere combined immunodeficiencydc.subject.mesh
TitleDevelopment and application of a novel in vivo EGFP based V(D)J recombination assaydc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-183dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-21211dc.identifier.urn
FacultyMedizinische Fakultätuulm.affiliationGeneral
Date of activation2002-10-23T16:02:01Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionZ: J-H 7.906 ; W: W-H 7.248uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS-ID2121uulm.vtsID
CategoryPublikationenuulm.category
University Bibliographyjauulm.unibibliographie


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