• English
    • Deutsch
View Item 
  •   OPARU Home
  • Medizinische Fakultät
  • Publikationen
  • View Item
  •   OPARU Home
  • Medizinische Fakultät
  • Publikationen
  • View Item
  • English 
    • English
    • Deutsch
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Rolle des C5a Rezeptors auf Neutrophilen Granulozyten während des Septischen Schocks im Menschen

Thumbnail
Download
vts_7220_10178.pdf (1.667Mb)
76 Seiten
 
Veröffentlichung
2010-03-01
DOI
10.18725/OPARU-2028

Dissertation

Authors
Schreiber, Heike Anja
Faculties
Medizinische Fakultät
License
Standard (ohne Print-On-Demand)
https://oparu.uni-ulm.de/xmlui/license_opod_v1
Abstract
Role of C5aReceptor (C5aR) on Neutrophils (PMN) during septic shock in humans: During experimental sepsis in animals, excessive generation of Complement 5a (C5a) and reduced binding on neutrophils (PMN) has been shown resulting in an impairment of the innate immune system with often lethal complications. To assess these results in humans, the present study analyzed complement status (levels of C3a, C5a and MAC) by Elisa and complement function by CH50. PMN function was assessed by detection of H202 production. C5aR expression and C5aR content of PMN were determined by Flow cytometry and Western blotting. A new technique for the detection of a so far unknown circulating C5aR (cC5aR) was developed. As a result patients with septic revealed an excessive generation of C3a, C5a and MAC and a significant reduction of complement function. The loss in complement function correlated with the outcome. PMN function was also significantly reduced in patients with septic shock also failed to distinguish between survivors and non-survivors. C5aR expression and C5aR content of PMN were significantly reduced during septic shock, especially in non-survivors. The loss of C5aR on PMN surface and lysate led to a discovery of a so far unknown circulating C5aR (cC5aR) in the sera. cC5aR levels were significantly elevated in patients with septic shock, especially in non-survivors. Using Roc analyses, C5aR and cC5aR were both reliable parameters to distinguish between survivor and non-survivor. In vitro incubation of PMN from healthy volunteers with CRP led to downregulation of C5aR expression and upregulation of cC5aR in the sera. Interestingly during septic shock sera CRP levels correlated with C5aR and cC5aR levels, thus indicating a crucial role for CRP in the regulation of C5aR and cC5aR. The data suggest that C5aR and cC5aR play a key role in the development and progression of septic shock in humans.
Date created
2008
Original publication
Adv.Exp.Med.Biol. 2006, titel: Complement activation during sepsis in humans
Subject Headings
Septischer Schock [GND]
Übertraining [GND]
Receptor, anaphylatoxin C5a [MeSH]
Shock, septic [MeSH]
Keywords
C5a Receptor (C5aR); Circulating C5a receptor (cC5aR)
Dewey Decimal Group
DDC 610 / Medicine & health

Metadata
Show full item record

Citation example

Schreiber, Heike Anja (2010): Rolle des C5a Rezeptors auf Neutrophilen Granulozyten während des Septischen Schocks im Menschen. Open Access Repositorium der Universität Ulm. Dissertation. http://dx.doi.org/10.18725/OPARU-2028

Other citation formats



About OPARU | Contact Us |
Impressum | Privacy statement
 

 

Advanced Search

Browse

All of OPARUCommunities & CollectionsFacultiesInstitutionsPersonsResource typesUlm SerialsDewey Decimal ClassesFundingThis CollectionFacultiesInstitutionsPersonsResource typesUlm SerialsDewey Decimal ClassesFunding

My Account

LoginRegister

Statistics

View Usage Statistics

About OPARU | Contact Us |
Impressum | Privacy statement