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AutorEdenfeld, Teresadc.contributor.author
Aufnahmedatum2016-03-15T06:24:30Zdc.date.accessioned
In OPARU verfügbar seit2016-03-15T06:24:30Zdc.date.available
Jahr der Erstellung2009dc.date.created
ZusammenfassungChromogranin A is a member of the granin family of acidic secretory glycoproteins that is found in secretory granules of many endocrine cells including neuroendocrine tumour cells. This hormone serves as a model system for autonomous hormone secretion by the so called functional neuroendocrine tumours of the gastrointestinal tract. The precise regulation of chromogranin secretion at the level of the Golgi apparatus is a subject of intense research. The protein kinase D (PKD) family of serine threonine kinases has so far been implicated in the regulation of constitutive secretion in epithelial cells. Here we examined whether PKD2 expression and activity could also play a role in the release of secretory granules from the trans Golgi network (TGN) in neuroendocrine tumour cells and hence be a target to block autonomous secretion by these tumours. Our data show that expression and catalytic activity of PKD2 are required for the release of chromogranin A containing secretory vesicles. Inhibition of PKD2 activity or siRNA knockdown of PKD2 resulted in a marked perinuclear retention of chromogranin A immunofluorescence in the trans Golgi network and led to a marked reduction in basal as well as phorbol ester stimulated secretion of chromogranin A into the supernatant of cells. Thus, PKD2 controls the release of secretory granules in neuroendocrine tumour cells at the level of the Golgi apparatus and could hence serve as a novel target to block hormone secretion in functional neuroendocrine tumours.dc.description.abstract
Sprachededc.language.iso
Verbreitende StelleUniversität Ulmdc.publisher
LizenzStandard (Fassung vom 01.10.2008)dc.rights
Link zum Lizenztexthttps://oparu.uni-ulm.de/xmlui/license_v2dc.rights.uri
SchlagwortProteinkinase D2dc.subject
DDC-SachgruppeDDC 610 / Medicine & healthdc.subject.ddc
MeSHProtein Kinase D2dc.subject.mesh
MeSHProtein kinasesdc.subject.mesh
TitelCharakterisierung der Funktion der Proteinkinase D2 als Regulator der Chromogranin A-Sekretion in humanen BON neuroendokrinen Tumorzellendc.title
RessourcentypDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-2016dc.identifier.doi
PPN318115581dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-71956dc.identifier.urn
GNDChromogranin Adc.subject.gnd
GNDNeuroendokriner Tumordc.subject.gnd
FakultätMedizinische Fakultätuulm.affiliationGeneral
Datum der Freischaltung2010-01-28T15:03:29Zuulm.freischaltungVTS
Peer-Reviewneinuulm.peerReview
Signatur DruckexemplarZ: J-H 13.547; W: W-H 11.982uulm.shelfmark
DCMI MedientypTextuulm.typeDCMI
VTS-ID7195uulm.vtsID
KategoriePublikationenuulm.category


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