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AuthorKuban, Jasmindc.contributor.author
Date of accession2016-03-15T06:24:26Zdc.date.accessioned
Available in OPARU since2016-03-15T06:24:26Zdc.date.available
Year of creation2008dc.date.created
AbstractThe C3 transferases from Clostridium botulinum (C3bot1) and Clostridium limosum (C3lim) ADP-ribosylate and thereby inactivate Rho A, B and C of eukaryotic cells. Cell accessibility of C3 proteins is very poor and no binding/translocation domain was identified for C3 proteins up to date. According to the current opinion, C3 proteins are taken up by unspecific pinocytosis when relatively high concentrations of C3 (> 30 µg/ml) were administered to cells for about 24 hours. Here, we show that relatively low concentrations of C3bot1 and C3lim (< 300 ng/ml) selectively intoxicated mouse J774A.1 macrophage-like cells. Both C3 transferases changed the morphology of J774A.1 cells in a concentration and time dependent manner. C3 bot1 and C3lim catalyzed the ADP-ribosylated of Rho in the cytosol, clearly indicating that the C3 proteins have been internalized into J774A.1. Enzymatic inactive C3bot1E174Q had no effect on J774A.1 cells; thus the ADP-ribosylation of Rho in the cytosol was essential to induce the observed C3-morphology. C3bot1 showed a concentration dependent binding to J774A.1 cells and competition studies with C3bot1E174Q revealed that the specific was binding. Bafilomycin A1, an inhibitor of the vacuolar ATPase proton pump, protected J774A.1 cells from intoxication with C3bot1 and C3lim. Data thus obtained imply that both C3 proteins translocate from acidified endosomes into the cytosol of cultured macrophage-like cells.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandard (Fassung vom 01.10.2008)dc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v2dc.rights.uri
KeywordC3dc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHADP ribose transferasedc.subject.mesh
MeSHBotulinum toxinsdc.subject.mesh
TitleCharakterisierung der Bindung und Aufnahme von clostridialen C3-Exoenzymen bei kultivierten makrophagenartigen Zellendc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-1993dc.identifier.doi
PPN614964717dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-71327dc.identifier.urn
GNDADP-Ribosyltransferasendc.subject.gnd
GNDClostridium botulinumdc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
Date of activation2009-12-01T15:18:07Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionZ: J-H 13.481; W: W-H 11.916uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS ID7132uulm.vtsID
CategoryPublikationenuulm.category
Bibliographyuulmuulm.bibliographie


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