Show simple item record

AuthorLorenz, Steffendc.contributor.author
Date of accession2016-03-15T06:23:45Zdc.date.accessioned
Available in OPARU since2016-03-15T06:23:45Zdc.date.available
Year of creation2011dc.date.created
AbstractThe influence of the polymer of the nanoparticle on the uptake in the cell was investigated with methacrylate nanoparticles with different polymer side chains, but equal in other particle characteristics (size, surfactant load). The influence of the Tg of the particles plays a crucial role on the cellular uptake of the particles. Particles with a glass transition point below the cell incubation temperature showed a better uptake in cells. The influence of functionalization was investigated with phosphonate functionalized particles in cell culture. The uptake, toxicity and the influence on the differentiation of stem cells was investigated closely in this thesis. MSC were differentiated in the presence of particles to the osteogenic, chondrogenic and adipogenic lineage. There was no toxicity and influence on the differentiation potential of the MSC in presence of the particles even after more than one month. Phosphonate groups allow the binding of particles to titanium surfaces. Coated surfaces were preferred for growth of MSC. Degradable capsules allow the disposal of active components in the cell. PBCA capsules containing fluorescent labeled oligonucleotides were used. It could be shown that these capsules were efficiently token up by the cells and didn’t show any toxic effect in cells. The PBCA capsules are able to release their load in cells. In this study we discovered the staining of mitochondria by CY5-labeled oligonucleotides. This was compared to commercial staining methods. Indeed the staining of CY5-labelled oligonucleotides took a little bit longer than the commercial one, but it could be used during a long time observation of cells without a negative influence on the cells. This method created a new cost-efficient alternative in the growing important sector of fluorescence analysis of cells. In addition it is a good and easy method to stain mitochondria over long time periods without any toxic effects.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandard (Fassung vom 01.10.2008)dc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v2dc.rights.uri
KeywordMitochondria stainingdc.subject
KeywordMitochondrienfärbungdc.subject
KeywordNanocapsulesdc.subject
KeywordTitan implantsdc.subject
KeywordTitanimplantatedc.subject
Dewey Decimal GroupDDC 570 / Life sciencesdc.subject.ddc
LCSHMitochondriadc.subject.lcsh
MeSHNanoparticlesdc.subject.mesh
MeSHStem cellsdc.subject.mesh
TitleInteraktion von Nanopartikeln mit immortalisierten Zelllinien und Stammzellendc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-1905dc.identifier.doi
PPN1650899793dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-75887dc.identifier.urn
GNDMitochondriumdc.subject.gnd
GNDNanokapseldc.subject.gnd
GNDNanopartikeldc.subject.gnd
GNDStammzelledc.subject.gnd
FacultyFakultät für Naturwissenschaftenuulm.affiliationGeneral
Date of activation2011-04-18T13:56:25Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionZ: J-H 14.147; W: W-H 12.611uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS ID7588uulm.vtsID
CategoryPublikationenuulm.category
Bibliographyuulmuulm.bibliographie


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record