Untersuchung des Einflusses verschiedener Ecdysone Response Elements auf die Stabilität und transkriptionelle Aktivität des Ecdysteroidrezeptors
FacultiesFakultät für Naturwissenschaften
LicenseStandard (Fassung vom 01.10.2008)
Nuclear receptors are ligand-dependent transcription factors, that regulate the expression of hormone inducible genes. I investigated the influence of different ecdysone response elements (EcREs) on the stability and transcriptional activity of the ecdysteroid receptor. The ecdysteroid receptor is the phylogenetically oldest steroid receptor and is formed by the ecdysone receptor (EcR) and ultraspiracle (Usp). The EcR / Usp heterodimer is distinguished as the functional ecdysteroid receptor, but EcR and Usp are also functional on their own. DNA-binding of the ecdysteroid receptor regulates not only its transcriptional activity, but also its protein concentration. Proteosomal degradation of the ecdysteroid receptor is possible, because degradation sequences - like ubiquitination sites or PEST sequences - are present in EcR and Usp. The transcriptional activity of the ecdysteroid receptor depends on the kind and number of the EcRE and is modulated by the A/B domains of EcR and Usp, as well as the DNA-binding domain (DBD) of Usp. Ecdysone response elements are one possibility how the homo- and heterodimers of the ecdysteroid receptor are able to regulate different procedures in the animal.
Subject HeadingsEcdysone [GND]
Drosophila melanogaster [LCSH]
Nuclear receptors [LCSH]
Transcriptional activation [MeSH]